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A method for identifying natural heterozygotes in kiwifruit

An identification method and technology for kiwifruit, which are applied in the direction of determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of difficulty in identification of natural heterozygotes of kiwifruit, and achieve the effect of avoiding data analysis difficulties, reducing influence and easy operation.

Inactive Publication Date: 2016-03-23
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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Problems solved by technology

[0004] Previous studies have shown that in wild populations of kiwifruit plants, the occurrence of natural hybridization often involves reticulated gene flow from more than two parents, which poses further difficulties for the identification of natural heterozygotes in kiwifruit

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  • A method for identifying natural heterozygotes in kiwifruit
  • A method for identifying natural heterozygotes in kiwifruit
  • A method for identifying natural heterozygotes in kiwifruit

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Embodiment Construction

[0026] Below in conjunction with accompanying drawing and embodiment describe in detail:

[0027] 1. Method

[0028] 1. If figure 1 Step ① Collect natural heterozygous samples to be identified and putative control parents. According to the geographical distribution of the existing species of Actinidia sinensis complexes, two varieties of Actinidia sinensis (A.chinensisvar.chinensis, AC) and delicious Actinidia (A.chinensisvar.deliciosa, AD) have different geographical distribution areas and ecological adaptability, Among them, the Chinese kiwifruit variety is distributed in the southeast of my country, and is mainly adapted to the middle and low altitude mountainous and hilly areas, while the delicious kiwifruit variety is mostly distributed in the central and western mountainous areas and higher-altitude ecological regions of my country. However, in the central and western parts of my country, there is a large geographical overlapping distribution area of ​​the two varieties...

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Abstract

The invention discloses a kiwi berry natural heterozygote identification method, and relates to the fields of plant genetic resource evaluation and the biotechnology. The method concretely includes the following steps: 1, collecting natural heterozygous samples to be identified and hypothetical control parents; 2, carrying out genome heredity analysis on the sample by using a microsatellite molecule marker; 3, constructing the microsatellite molecule phenotype database of all the samples; 4, determining a true control parent by using multiple correspondence analysis of the molecular phenotype data; and 5, identifying the natural heterozygote based on the control parent. Compared with traditional kiwi berry natural heterozygote identification methods, the kiwi berry natural heterozygote identification method has the characteristics of simple molecular experimental process, fast and efficient analysis and identification flow, and the like; the method can overcome the problems of difficult kiwi berry pure and control sample determination, difficult processing of complex data of genome ploidy, and the like; and the method also can mitigate the influences of genetic coancestry on the heterozygote identification.

Description

technical field [0001] The invention relates to the fields of plant genetic resource evaluation and biotechnology, in particular to a method for identifying natural heterozygotes of kiwifruit. It specifically involves the genetic evaluation of genome microsatellite molecular markers of natural heterozygotes and control parents within different species and between species of Actinidia, and uses improved molecular gene phenotype data processing and analysis methods to assist in determining the true control parents. Parameter-optimized analysis software screened and identified natural heterozygotes. Background technique [0002] Actinidia belongs to Actinidiaceae (Actinidiaceae) and is a perennial woody vine. The genus has 54 species and 21 varieties of about 75 taxa. Chinese kiwifruit (A.chinensis var. chinensis) and delicious kiwi fruit (A. chinensis var. deliciosa), two varieties of the Chinese kiwi fruit (A. .erianthaBentham), soft date kiwifruit (A.arguta (SieboldandZuc...

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q2600/156
Inventor 刘义飞黄宏文李大卫钟彩虹
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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