Method for coproducing D-arginine and gamatine through biotransformation

Abstract

The invention discloses a method for coproducing D-arginine and gamatine through a biological method. The method comprises the following steps: synthesizing arginine racemase genes argR according to a designed primer, establishing an expression vector pET24a-argR and transferring the expression vector into an Escherichia coli strain, thus obtaining genetically engineered bacteria of the arginine racemase; establishing an expression vector pET24a-speA through a speA gene segment amplified by PCR, transferring the expression vector pET24a-speA into Escherichia coli, thus obtaining L-arginine decarboxylase genetically engineered bacteria; stirring and reacting a transformation system containing L-arginine and a wet vector for expressing the genetically engineered bacteria of the arginine racemase, thus obtaining an enantiomer of L-arginine and D-arginine; removing the arginine racemase, supplementing a wet vector for expressing L-arginine decarboxylase, and continuously reacting until the L-arginine is completely consumed; and finally, separating and purifying the D-arginine and gamatine in the reaction system. The catalyzing enzyme activity is high, the separation and purification operations are simple, the raw materials are cheap, the total production cost is low; and the method has high conversion rate, can realize large-scale production and is suitable for industrial application.

Description

technical field [0001] The invention relates to a production method of D-arginine and agmatine, in particular to a method for co-producing D-arginine and agmatine through a biological method. Background technique [0002] D-arginine (D-arginine, D-arg), as the enantiomer of L-arginine, is a non-protein amino acid that exists in a small amount in the body. Studies have shown that D-arginine in organisms has unique physiological functions, such as inhibiting the proliferation of cancer cells, antihypertensive and so on. In addition, as a chiral component, D-arginine is also an important chiral reagent and pharmaceutical intermediate. [0003] D-arginine exists in a small amount in nature and is not easy to obtain through extraction. The current method is mainly to use chemical methods (such as salicylaldehyde method) to racemize L-arginine to obtain a mixture of DL-arginine, and then resolve it by chemical methods or biological enzyme catalysis to obtain chirally pure D - ...

AI Technical Summary

Problems solved by technology

However, what is currently used in industry is chemical production, which has many route steps, low yield and heavy pollution.

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