A method to enhance the mechanical properties of silk protein membranes through biomineralization

Abstract

The invention discloses a method for improving mechanical properties of a silk protein membrane by virtue of biological mineralization. The method is realized by the following steps of dissolving an aqueous solution of silk protein or silk protein powder in organic solvents such as hexafluoroacetone, hexafluoroisopropanol and formic acid to obtain a silk protein solution, then dropwises adding the aqueous solution of silk protein or the silk protein solution to a plastic plate and drying to obtain the silk protein membrane. By adopting the method disclosed by the invention. The mechanical properties of the silk protein membrane are significantly improved and the time is greatly saved and the method has the advantages of short production period, simple and efficient extraction process and no complicated and tedious mineralization process and is simple to operate.

Description

technical field [0001] The invention relates to a biomineralization method of a silk protein film, in particular to a biomineralization method for significantly improving the mechanical properties of the silk protein film, which belongs to a material processing method. Background technique [0002] Hydroxyapatite has good biocompatibility and is the main inorganic component of human bones and teeth. There are as many as tens of millions of patients with bone defects worldwide every year due to various factors such as trauma, disease, and genetics. Simulating the main components of natural bone, the bio-scaffold prepared by combining minerals and materials makes bone repair a possibility. By mineralizing natural polymer materials, scaffold materials with similar physicochemical properties to natural bone components can be prepared, which is also a commonly used method in the preparation of biological scaffold materials. [0003] Silk protein fibers such as silkworm silk pro...

AI Technical Summary

Problems solved by technology

However, common mineralization methods (physical or chemical mineralization) significantly reduce the mechanical properties of silk protein films, and incomplete mineralization is common, and impurities other than hydroxyapatite are easily generated, so it cannot meet the needs of scientific research and production. needs

For example, the publication date is July 04, 2012, and the Chinese patent with the publication number CN102532573A discloses a rapid prototyping method for sericin / hydroxyapatite composite film. Rapid prototyping of stone composite films, but easy to generate by-products of hydroxyapatite

[0004] To sum up, there is no biomimetic mineralization method with simple process, easy operation, pure mineralization, and improved mechanical properties of silk protein membranes.

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