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Method for extracting theabrownin extractive from liupu tea and application thereof

An extraction method and Liubao tea technology are applied in the application field of theabrownin in protecting the liver, which can solve the problems of low extraction rate and the like, and achieve the effect of improving the extraction rate.

Inactive Publication Date: 2014-11-19
GUANGXI NANXIANG ENVIRONMENTAL PROTECTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for extracting theabrownin in Liubao tea, which solves the problem of low extraction rate in the prior art

Method used

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  • Method for extracting theabrownin extractive from liupu tea and application thereof
  • Method for extracting theabrownin extractive from liupu tea and application thereof
  • Method for extracting theabrownin extractive from liupu tea and application thereof

Examples

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Effect test

Embodiment 1

[0050] A method for extracting theabrownin in Liubao tea, specifically implemented according to the following steps: Pulverize the tea leaves of Liubao tea with a pulverizer, pass through a 60-mesh sieve, and pass through a dose of 24kGy 60Co-γ-ray irradiation to obtain Liubao tea tea powder; weigh 10g Liubao tea tea powder, add 100ml of distilled water, the pH of the distilled water is 4, add 20mg of cellulase, hydrolyze at 30°C for 20min, Obtain the enzymolysis treatment solution; ultrasonically treat the enzymolysis treatment solution at room temperature for 30 minutes to obtain the ultrasonic treatment solution; heat the ultrasonic treatment solution to a temperature of 100°C, reflux and extract for 40 minutes, suction filter the solution while it is hot, and then press the same volume as the above The amount of distilled water was added to distilled water for leaching 3 times, and the filtrate was combined; the above-mentioned filtrate was extracted with an equal volume of...

Embodiment 2

[0052] A method for extracting theabrownin in Liubao tea, which is specifically implemented according to the following steps: Pulverize the tea leaves of Liubao tea with a pulverizer, pass through a 40-mesh sieve, and pass through a dose of 2kGy 60 Co-γ-ray irradiation to obtain Liubao tea tea powder; weigh 10g Liubao tea tea powder, add 80ml of distilled water, the pH of the distilled water is 2, add 5mg of cellulase, hydrolyze at 30°C for 20min, Obtain the enzymolysis treatment solution; ultrasonically treat the enzymolysis treatment solution at room temperature for 30 minutes to obtain the ultrasonic treatment solution; heat the ultrasonic treatment solution to a temperature of 80°C, reflux and extract for 20 minutes, suction filter the solution while it is hot, and then press the same volume as above The amount of distilled water was added to distilled water for leaching 3 times, and the filtrate was combined; the above-mentioned filtrate was extracted with an equal volume ...

Embodiment 3

[0054] A method for extracting theabrownin in Liubao tea, which is specifically implemented according to the following steps: pulverize the tea leaves of Liubao tea with a pulverizer, pass through a 70-mesh sieve, and pass through a dose of 20kGy 60 Co-γ-ray irradiation to obtain Liubao tea tea powder; weigh 10g Liubao tea tea powder, add 120ml of distilled water, the pH of the distilled water is 10, add 30mg of cellulase, hydrolyze at 60°C for 100min, Obtain the enzymolysis treatment solution; ultrasonically treat the enzymolysis treatment solution at room temperature for 30 minutes to obtain the ultrasonic treatment solution; heat the ultrasonic treatment solution to a temperature of 90°C, reflux and extract for 60 minutes, suction filter the solution while it is hot, and then press the same volume as above The amount of distilled water was added to distilled water for leaching 3 times, and the filtrate was combined; the above-mentioned filtrate was extracted with an equal vo...

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Abstract

The invention discloses a method for extracting theabrownin extractive from liupu tea. The method for extracting theabrownin extractive from liupu tea comprises the following steps: pre-irradiating raw materials; processing by using an enzymic method; processing by using ultrasound wave; heating and extracting; extracting chloroform; extracting by using ethyl acetate; extracting by using n-butyl alcohol; and drying an obtained product by distilling to obtain the theabrownin extractive. The invention also provides an application of the theabrownin extracted by the method for protecting liver. Due to the adoption of irradiation of 60Co-gamma rays, the oxidative polymerization of the tea polyphenol substances can be facilitated to form more theabrownin; due to the adoption of a cellulase auxiliary extraction technology, the extraction rate of the theabrownin in a solvent method can be remarkably increased; the theabrownin extractive with the content of about 25% can be obtained; compared with other methods, the extraction rate of the method is 16% more than that of the other method; the theabrownin is considered to have a certain protection action on the acute drug-induced liver injury in the liver protection experiment and can be used as development of foods with liver protection functions; the theabrownin has a certain application prospect.

Description

technical field [0001] The invention belongs to the technical field of food processing, and specifically relates to a method for extracting theabrownin extract from Liubao tea, and also relates to an application of the theabrownin extracted by the above method in protecting the liver. Background technique [0002] Liubao tea is one of the six major dark teas. It is originally produced in Liubao Township, Cangwu County, Wuzhou City, Guangxi. It has a long history and good quality. Known as the famous historical tea. Its production history can be traced back to 1,500 years ago. During the Jiaqing period of the Qing Dynasty, it was listed as one of the twenty-four famous teas in China due to its own special betel nut fragrance. Cheng Yuandao in the late Qing Dynasty once wrote a poem to praise Liubao tea: "Liubao famous tea is full of mountains and hills, and it is good at relieving ailments and greasy. Please make a bowl and drink it tonight, and the fragrance of teeth and ch...

Claims

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Application Information

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IPC IPC(8): A23F3/16
Inventor 何英姿朱家安王晓林乃杰刘道权吴永平
Owner GUANGXI NANXIANG ENVIRONMENTAL PROTECTION
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