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Method for establishing coronary artery microvascular spasm pig-model

A technology of coronary arteries and microvessels, applied in the field of medical models, can solve the problems of difficult experimental research of models, long modeling cycle, and large trauma, and achieve the effect of short modeling time, high incidence rate, and less trauma

Inactive Publication Date: 2014-11-05
THE AFFILIATED HOSPITAL OF SHANDONG UNIV OF TCM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the above-mentioned models are located in the large epicardial blood vessels, and cannot specifically cause small and microvascular spasm, which has a certain gap with clinical psychological stress and other characteristics. Moreover, the operation is complicated, traumatic, and the modeling period is long. Therefore, these models are difficult to be widely used in experimental research

Method used

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  • Method for establishing coronary artery microvascular spasm pig-model
  • Method for establishing coronary artery microvascular spasm pig-model
  • Method for establishing coronary artery microvascular spasm pig-model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: 1nmol group

[0042] Select 4 of the above-mentioned miniature pigs, aged 6 months, weighing 28-37Kg, containing 10ml of normal saline-neuropeptide Y injection containing 1nmol neuropeptide Y, and the specific steps are as follows

[0043] 1) Animal handling

[0044] The pigs were fasted for 8 hours. Before the test, the hair was removed from the chest and femoral arteries on both sides, and the body weight was measured. Anesthesia was induced by intramuscular injection of 10 mg / kg ketamine, tracheal intubation, ventilator assisted breathing, and electrocardiogram was connected; Intravenous access was established at the site, and 5 ml of 3% pentobarbital sodium was slowly injected intravenously, and oxygen was given at a flow rate of 3 L / min. Anesthesia was maintained during the operation, and 2ml of pentobarbital sodium was added every 1h or administered depending on the movement of limbs.

[0045] 2) Intracoronary injection of saline and neuropeptide Y: ...

Embodiment 2

[0047] Example 2: 3nmol group

[0048] Select 4 above-mentioned miniature pigs, age 6 months, body weight 28-37Kg, physiological saline-neuropeptide Y injection containing 3nmol neuropeptide Y 10ml, the steps are the same as in Example 1.

Embodiment 3

[0049] Example 3: 6nmol group

[0050] Select 4 above-mentioned miniature pigs, age 6 months, body weight 28-37Kg, 10ml of physiological saline-neuropeptide Y injection containing 6nmol neuropeptide Y, the steps are the same as in Example 1.

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Abstract

The invention provides a method for establishing a coronary artery microvascular spasm pig-model, and belongs to the technical field of medical models. The method comprises that after bilateral femoral arteries of a test pig are free, distal ends of the femoral arteries are ligatured by wires, a left-side sheathing canal is fed into a pigtail catheter, blood flow dynamics indexes are determined, in a right-side sheathing canal, a coronary artery radiography process is carried out so that the middle of an anterior descending branch is displayed, and a neuropeptide Y is injected into the coronary artery so that the coronary artery microvascular spasm pig-model is obtained. The coronary artery microvascular spasm pig-model has good singularity, well fits to the clinical disease fact, satisfies clinical psychological stress characteristics, can be operated simply, is scientific and reasonable, has short modeling time, small damage and low animal mortality, realizes coronary artery microvascular evaluation on the carrier, realizes qualitative and quantitative evaluation of microvascular spasm state and degree, realizes real-time observation, provides a novel research means for basic clinical research and is suitable for popularization application.

Description

technical field [0001] The invention belongs to the technical field of medical models, and relates to a method for establishing a pig model of coronary microvascular spasm. Background technique [0002] Coronary artery spasm-plaque rupture-thrombosis-acute coronary event is the main mechanism of coronary heart disease. Coronary artery spasm (CAS) refers to the segmental or diffuse spastic contraction of coronary smooth muscle caused by various reasons, causing myocardial ischemia and even myocardial necrosis. Clinically, CAS is not only the main cause of variant angina, but also closely related to acute myocardial infarction, malignant arrhythmia and sudden cardiac death. Recent studies have shown that CAS can occur not only in those with damaged coronary arteries, but also in those with normal coronary arteries. Even if there is no significant stenosis of large vessels, recurrent spasm of microvessels and small vessels can increase the short-term and long-term inciden...

Claims

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Application Information

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IPC IPC(8): A61K38/17
Inventor 李晓姜萍林海青
Owner THE AFFILIATED HOSPITAL OF SHANDONG UNIV OF TCM
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