Method for detecting CA6 virus with real-time fluorescent quantitative PCR

Abstract

The invention belongs to the field of nucleic detection and relates to a method for detecting CA6 virus with real-time fluorescent quantitative PCR. According to the method, a specific primer and a Taqman probe are designed on the basis of a CA6VP1 sequence and are used for detecting the CA6 virus rapidly, specifically and high-efficiently. By means of the method, gene segment of CA6 can be stably and effectively amplified and a highest detection sensitivity can reach 10 copius / [mu]l. In addition, the method is high in specificity and no false positive result is detected. The method for detecting the CA6 is quick, sensitive and reliable, can be further used for laboratory detection of a large-scale sample and an epidemiological inspection operation of the CA6 and is helpful to early-stage quick diagnosis in clinical practice.

Description

technical field The invention belongs to the detection field and relates to a nucleic acid detection method, in particular to a method for real-time fluorescent quantitative PCR detection of CA6 virus. Background technique The prior art discloses that hand, foot and mouth disease (HFMD) is a common infectious disease caused by human enterovirus (HEV); statistics show that the disease can occur all year round, 5 From July to July is the high-incidence period, and it is more common in children under 5 years old. Most of them have mild clinical symptoms, mainly fever and rash or herpes on the hands, feet, mouth and other parts. A small number of patients may have aseptic meningitis, encephalitis, Inflammation, acute flaccid paralysis (AFP), neurogenic pulmonary edema, and myocarditis, etc., the disease progresses rapidly in some severely ill children and can lead to death. Since 1997, there have been several outbreaks of HFMD in Malaysia, Singapore, Australia, China and other ...

AI Technical Summary

Problems solved by technology

So far, there have been several outbreaks of HFMD caused by CA6 around the world, but there are few reports on rapid and sensitive methods for specific detection of CA6, especially no specific real-time PCR (real-time PCR) detection Report on the method of CA6 virus

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