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Lemna Minor Hydrolysate And Use Thereof

A hydrolyzate and duckweed technology, applied in biochemical equipment and methods, microorganisms, methods based on microorganisms, etc., can solve problems such as poor yield and high price

Inactive Publication Date: 2014-10-15
LEMNACEAE FERMENTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when utilizing microorganisms to manufacture such products, many problems are still encountered
For example, the ingredients used in the medium are expensive and may yield poorly in large-scale fermentations

Method used

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  • Lemna Minor Hydrolysate And Use Thereof
  • Lemna Minor Hydrolysate And Use Thereof
  • Lemna Minor Hydrolysate And Use Thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0032] The preparation of example 1 duckweed hydrolyzate

[0033] Get 100kg of fresh duckweed (Qingping), wash it with a grinder or process it with a juicer. The liquid fraction was collected and filtered to obtain duckweed juice. The obtained duckweed juice (60 L) was treated with 450 A.U. of Protamex at 40° C. for 20 hours to obtain duckweed hydrolyzate.

example 2

[0034] Example 2 prepares lycopene without using duckweed hydrolyzate

[0035] (1) Microorganisms:

[0036] The Escherichia coli CCRC940321 used to prepare lycopene was constructed according to the method disclosed in US7,122,341B1. Briefly, plasmid pCW9 and p2IDEI were introduced into Escherichia coli JCL1613 to obtain lycopene-producing host cells. Escherichia coli CCRC940321 was obtained from the Institute of Food Industry Development (No. 331 Food Road, Hsinchu City, Taiwan).

[0037] (2) Medium:

[0038] Inoculum culture medium:

[0039] 2% (w / v) yeast extract (Difico)

[0040] 2% (w / v) tryptone (Difico)

[0041] 2% (w / v) Glycerin (Sigma)

[0042] Batch medium: (2L)

[0043]

[0044]

[0045] Feed medium: (1L)

[0046]

[0047] (3) Method:

[0048] Add frozen Escherichia coli CCRC940321 to a 250 mL culture shaker flask containing 50 mL of inoculum culture medium, and culture at 32°C for 12 hours with shaking at 150 rpm. The activated cells were transfer...

example 3

[0049] Example 3 is using duckweed hydrolyzate to prepare lycopene

[0050] (1) Microorganisms:

[0051] Escherichia coli CCRC940321

[0052] (2) Medium:

[0053] Inoculum culture medium:

[0054] 2% (w / v) yeast extract (Difico)

[0055] 2% (w / v) tryptone (Difico)

[0056] 2% (w / v) Glycerin (Sigma)

[0057] Batch medium: (2L)

[0058]

[0059] Feed medium: (1L)

[0060]

[0061] (3) Method:

[0062] Add frozen Escherichia coli CCRC940321 to a 250 mL culture shaker flask containing 50 mL of inoculum culture medium, and culture at 32°C for 12 hours with shaking at 150 rpm. The activated cells were transferred to a 5L-fermenter containing 2L batch medium and cultured at 30°C with 400-600rpm agitation and 2L / min aeration. When the cell density (OD600) reaches 15 to 25, the feed medium is added to the fermenter (pH value is controlled at 6.8-7.0). After 108 hours, the cells were harvested at a cell density (OD600) of 190 (equivalent to 62.7 g stem cells per liter of...

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PUM

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Abstract

The present invention provides a lemna minor hydrolysate, and a method of using the lemna minor hydrolysate to cultivate microorganism to prepare carotenoid.

Description

technical field [0001] The present invention relates to the hydrolyzate of duckweed, which can be used as a nutrient for culturing microorganisms such as Escherichia coli to increase cell biomass and yield of cell products. Background technique [0002] Duckweed (which includes plants of the Lemnaceae family) is known to have a rapid doubling time and has also been widely used as feed for ducks, chickens, fish and shrimp. Duckweed is also used in wastewater treatment and can convert carbon dioxide into biomass. Therefore, Xian believes that duckweed has considerable potential in solving serious water and weather problems. Cheng et al. (Growing Duckweed to recover nutrients from wastewaters and for production of fuel ethanol and animal feed. Clean. 37 (1). 17-26 (2009)) revealed that duckweed can remove nitrogen and phosphorus from wastewater, and floating Ping Ping biomass can be used as an alternative source of carbohydrates required for fermentation in fuel ethanol manuf...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C12P5/02C12R1/19C12R1/18C12R1/01
Inventor 邱明熙
Owner LEMNACEAE FERMENTATION
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