Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extracting selenocystine and selenomethionine from selenium-gathered plant Cardamine hirsuta

A technology of selenocystine and selenomethionine, which is applied in food science, metabolic diseases, drug combination, etc., can solve problems such as waste of resources, and achieve the effects of high product extraction rate, simple technical process, and wide application

Active Publication Date: 2014-10-08
恩施硒谷科技股份有限公司
View PDF1 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Enshi State Selenium Research Institute found that the broken rice chestnut not only contains high selenocystine, but also has a certain amount of selenomethionine. It is a biological selenium resource with high utilization value. Although it can be eaten directly, Since it has not passed food certification, it cannot be added to food, resulting in a huge waste of resources

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting selenocystine and selenomethionine from selenium-gathered plant Cardamine hirsuta
  • Method for extracting selenocystine and selenomethionine from selenium-gathered plant Cardamine hirsuta

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The present invention is a method for extracting selenocystine and selenomethionine from the polyselenium plant Cardamine. The specific steps are as follows: weigh 10g of a sample of Cardamine with a selenium content of 2158.97 mg / kg, and add 50 mL of ultrapure Water, sonicate for 60min, filter, and extract the filter residue twice with water. Wash the residue, combine the filtrate, and dilute to 200mL. Detected by HPLC-HG-AGS. The chromatographic conditions are: anion exchange column, A mobile phase 40mM, B mobile phase 60mM (Adjust the pH value to 6.0 with formic acid), flow rate 1.0mL / min, use 0min~3min: 100% A; 3min~4min: 100% A→100% B; 4min~10min: 100% B; 10min~12.5min: 100% A gradient elution, retention time of selenocystine is 2.42±0.25min, and the content of selenocystine in the extract is 952.25mg / kg. It is separated by high-pressure preparation system, and the conditions are the same as the above chromatogram. 2. For the solution between 10 and 2.70 minutes, pu...

Embodiment 2

[0053] The present invention is a method for extracting selenocystine and selenomethionine from the polyselenium plant Cardamine. The specific steps are as follows: Weigh 10g of a sample of Cardamine with a selenium content of 2158.97 mg / kg, and add 50mL0.1mol / Shake the L HCl solution for 90 min at 60°C, extract the filter residue twice with water, wash the residue, combine the filtrate, adjust the pH value to 6-7 with NaOH solution, and dilute to 200 mL. Detected by HPLC-HG-AGS. The content of selenocystine is 747.00mg / kg, and the content of selenomethionine is 216.34mg / kg. It is separated by a high-pressure preparation system under the same conditions as the above chromatogram. The selenocystine solution between 2.10 and 2.70 min is collected in sections And the selenomethionine solution between 3.10 and 3.70 minutes, dialyzed, freeze-dried and weighed, respectively 5.35mg and 1.88mg, the purity was 98.4% and 98.8% respectively.

Embodiment 3

[0055] The present invention is a method for extracting selenocystine and selenomethionine from the polyselenium plant Cardamine. The specific steps are as follows: Weigh 10g of a sample of Cardamine with a selenium content of 2158.97 mg / kg, and add 50mL 1mol / L HCl, ultrasonic extraction for 60 minutes, the filter residue was extracted twice, the residue was washed, the filtrate was combined, the pH value was adjusted to 6-7 with NaOH solution, the volume was adjusted to 200 mL, and the selenocystine in the extract was detected by HPLC-HG-AGS The content is 763.56 mg / kg, and the content of selenomethionine is 223.16 mg / kg. It is separated by a high-pressure preparation system under the same conditions as the above chromatography. The selenocystine solution and selenomethionine solution are collected in sections, and weighed after dialysis and freeze drying. The weight is 6.18mg and 1.95mg, and the purity is 98.5% and 98.6%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for extracting selenocystine and selenomethionine from selenium-gathered plant Cardamine hirsuta. The method comprises the following steps: after crushing a dried poly-selenium plant Cardamine hirsute material, extracting selenocystine by adding hydrochloric acid, extracting selenomethionine by adding sodium hydroxide, regulating the pH value of a filtrate to be nearly neutral, separating by using a high pressure preparation column, removing inorganic salt ions of an eluent containing selenocystine and selenomethionine by respectively using cellulose ester dialysis bags, and then, carrying out freeze drying to obtain high-quality selenocystine and selenomethionine products. The technical process is simple; the product extraction rate is high; the produced selenocystine and selenomethionine products can act as crude drugs for producing injections or food additives, are wide in use, and meanwhile can promote the development of Cardamine hirsuta planting industry, so that the industrial chain is extended.

Description

Technical field [0001] The invention relates to a method for extracting selenocystine and selenomethionine from polyselenium plant Cardamine. Background technique [0002] Selenium is one of the essential trace elements for the human body. Selenocystine is the twenty-first amino acid essential for the human body. Selenomethionine replaces methionine to synthesize protein in the human body. More than 40 human diseases are related to selenium deficiency. It is a rare element with very little content on the earth, causing about 72% of the world to be short of selenium. There are more than 900 million people in China who need selenium supplementation. The annual demand for selenium is about 1,000 tons, of which 80% rely on imports. In the past, the most important way to supplement selenium for humans and animals was to add sodium selenite to food and feed, or to eat selenium-enriched yeast flakes for patients, but the harm of sodium selenite to the human body has been recognized by p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07C391/00A23L1/305A61K31/198A61P3/02
Inventor 陈永波胡百顺李红英黄光昱石月明
Owner 恩施硒谷科技股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com