Feed additive and preparation method thereof

A technology of feed additives and raw materials, applied in the field of high-efficiency feed additives and its preparation, can solve problems such as difficulty in realizing industrialization, standardization, impact on feed nutrition ratio, and simple production equipment, so as to enhance cellular immunity and humoral immunity, improve daily Weight gain and feed conversion ratio, the effect of less usage

Active Publication Date: 2014-10-08
BEIJING DORUN TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the Chinese herbal medicine feed additives put on the market are powders or powders. Their production technology is backward, the production equipment is simple, the processing is rough and simple, the variety is single, and the dosage is generally too large. This not only increases the product cost, wastes medicine, but also affects The nutrient ratio of the feed is not satisfied. The existence of these problems also makes the Chinese herbal medicine feed additives on the market do not meet the basic functional principle of "trace amount, high efficiency" feed additives, and it is difficult to realize industrialization and standardization.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0037] (1) Preparation of bacterial suspension

[0038] Inoculate the single colony of Li-2013 grown after streaking on the plate into the seed medium, culture at 100r / min, 40°C for 12h, take 1mL of the culture medium, centrifuge, wash twice with normal saline, and resuspend in 9mL of physiological in salt water.

[0039] (2) Ultraviolet-Lithium Chloride-Diethyl Sulfate Compound Mutagenesis

[0040] The bacterial suspension was placed on a sterile plate, stirred and irradiated for 100s under a UV lamp with a distance of 30cm and a power of 15w. The irradiated bacteria solution was diluted and spread on the lithium chloride plate after gradient dilution, and the bacteria solution without ultraviolet irradiation was diluted and spread on the plate as a control. Wrap the uniformly coated plate above with black cloth or newspaper, incubate at 40°C for 48 hours, screen out the plate with the largest ratio of hydrolysis circle to colony diameter on the plate that grows colonies, p...

Embodiment 1

[0057] Embodiment 1: preparation method is the same as example 3

[0058] A feed additive comprises the following raw materials in parts by weight: 27 parts of astragalus extract; 15 parts of Codonopsis pilosula extract; 16 parts of Bupleuri extract; 20 parts of Bacillus subtilis culture.

Embodiment 2

[0060] Preparation of Bacillus subtilis culture:

[0061] Obtain Bacillus subtilis fermentation broth by step-by-step expansion culture of slant strains;

[0062] (1) Primary seed culture: Inoculate the slant strain of Bacillus subtilis into a 500-ml shake flask, with a medium volume of 100 ml, a rotary shaker at 180 rpm, a culture temperature of 40°C, and a culture time of 12 hours;

[0063] (2) secondary seed cultivation: the primary seed is inserted into a 500 milliliter secondary seed shake flask according to the inoculum size of 10%, and the cultivation condition is the same as that of the primary seed;

[0064] (3) Tertiary seed cultivation: Inoculate the secondary seeds with 10% inoculum amount into a 5000 ml tertiary seed shake flask, the medium loading capacity is 1000 ml, the rotary shaker is 100 rpm, and the cultivation temperature is 40°C. Time 12 hours;

[0065] (4) First-level seed tank cultivation: put the third-level seeds into the first-level seed tank with ...

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PUM

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Abstract

Belonging to the field of feed additives, the invention discloses a feed additive and a preparation method thereof. The feed additive comprises the following raw materials by weight: 20-35 parts of an astragalus extract; 10-20 parts of a Codonopsis pilosula extract; 10-20 parts of a radix bupleuri extract; and 10-25 parts of a bacillus subtilis culture. The product provided by the invention has the characteristics of small usage, stable performance, use safety, and no incompatibility with other feed additives. The Chinese herbal extracts and a microbial agent are adopted in a reasonable proportion, enzymolysis and ethanol immersion extraction are employed to improve the extraction rates of amino acid, polysaccharide, trace elements and other beneficial ingredients in Chinese herbal medicines, and then by combining with the bacillus subtilis culture with high enzyme yield, digestion and absorption of beneficial Chinese herbal ingredients in feed can be promoted effectively. At the same time, the usable value of energy and protein of various feed resources is also enhanced, the production ability, the stress response and raising comprehensive benefits of livestock and poultry are strengthened, the animal daily gain and feed conversion rate are improved, and the drug usage is saved.

Description

Technical field: [0001] The invention belongs to the field of feed additives, in particular to a high-efficiency feed additive containing various traditional Chinese medicine components and a preparation method thereof. Background technique: [0002] With the rapid development of animal husbandry in our country, the application of feed additives has become more and more extensive, which has made great contributions to ensuring the healthy development of animal husbandry and meeting people's demand for animal food, but it has also brought a series of problems. Since most of the additives belong to chemically synthesized drugs, such as hormones, antibiotics, etc., while increasing the production of livestock and poultry, the drug residues in livestock products increase, which directly endangers human health; and in the process of modern livestock and poultry breeding, in order to prevent And to treat animal diseases, excessive antibiotic products are often used to ensure anima...

Claims

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Application Information

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IPC IPC(8): A23K1/16A23K1/14A23K1/00A23K10/12A23K20/10A23K20/189
Inventor 朱建国王剑顾学珠夏圣荣伍华东
Owner BEIJING DORUN TECH
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