Molecular marker of rice-blast-resistant gene Pi2 and application of molecular marker

Abstract

The invention belongs to the field of crop biotechnology and in particular relates to a molecular marker of a rice-blast-resistant gene Pi2 and application of the molecular marker. The upstream and downstream molecular markers are based on specific insertion / deletion loci polymorphism of nucleotide sequences shown in SEQ ID NO.1 and SEQ ID NO.2. The two molecular markers are co-dominant molecular markers developed for close linkage regions on two sides of the functional gene Pi2, and have the advantages of being high in accuracy and capable of being widely applied to different breeding parents. By detecting the two molecular markers, whether a detected rice material has the rice-blast-resistant gene Pi2 or not can be accurately judged. By virtue of a molecular marker combination, whether a hybrid transferred progeny plant genome of a parent of the functional gene Pi2 and other parents has the functional gene Pi2 or not and the homozygous state of the functional gene Pi2 can be accurately detected, and the breeding efficiency of a rice-blast-resistant rice variety with the functional gene Pi2 is improved.

Description

Technical field [0001] The present invention belongs to the field of crops and biotechnology, which involves rice resistance genes PI2 Molecular marks and applications. Background technique [0002] Rice is one of the most important grain crops in China and even the world, but its production is severely harmful to rice plague.In the world, the rice plastic bacteria ( Magnapotheezae ) The cause of rice plague can cause losses of 10-30%of rice output every year (Dean et al., 2005).Long -term production practice has shown that selection and use of disease -resistant rice varieties are the most effective, economical, and environmental safety methods to prevent and treat rice plague. [0003] The selection of traditional disease -resistant rice varieties mainly depends on the evaluation of the seedling inoculation phenotype of the seedling stage or the anti -episode form in the rice plastic disease is easy to be screened.The identification results are inaccurate and the breeding effic...

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Problems solved by technology

[0003] The breeding of traditional disease-resistant rice varieties mainly relies on the phenotypic identification of resistance inoculation at the seedling stage or the screening of resistance phenotypes in rice blast-prone areas, which is easily affected by changes in the environment, climate and other conditions of rice blast occurrence, resulting in Inaccurate identification results and low breeding efficiency

In addition, there is phenotypic overlapping of resistance spectrum among many disease resistance genes. Traditional breeding relies on phenotypic selection, and it is difficult to identify long-lasting broad-spectrum disease-resistant varieties that aggregate multiple disease-resistant genes.

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