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Molecular marker interlocked with cotton fiber length major QTL and application thereof

A technology of molecular markers and fiber length, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of slow progress, poor accuracy, and long cycle of fiber length breeding, and achieve faster breeding and seed breeding. The process of industrialization, the solution to the slow progress, and the effect of improving fiber quality

Active Publication Date: 2018-12-21
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to overcome many shortcomings such as poor accuracy of phenotypic selection, low efficiency, long cycle and high cost in traditional breeding, and solve the problem of slow progress in fiber length breeding

Method used

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  • Molecular marker interlocked with cotton fiber length major QTL and application thereof
  • Molecular marker interlocked with cotton fiber length major QTL and application thereof
  • Molecular marker interlocked with cotton fiber length major QTL and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Screening molecular markers

[0040] (1) Group construction and data acquisition

[0041] Using Haidao Mianhai 1 as the donor parent and Zhongmiansuo 45 (Zhong 45) as the recurrent parent, cross backcross and self-cross to obtain Zhongmiansuo 45×Hai 1BC 4 f 3：5 Chromosomal segment substitution lines were evaluated in multiple environments (Ma Liujun et al., 2013) and molecular genotype detection [markers from molecular genetic linkage map (Shi et al. Substitution system MIBI7747 ( figure 1 ) as the female parent, crossed with the reincarnated parent Zhongmiansuo 45 as the male parent, and constructed a secondary segregation large population BC 5 f 2 .

[0042] Planting BC in Anyang in 2013 5 f 2 A large population of 900 plants was isolated, with a row length of 8 m, a row width of 80 cm, and a plant spacing of 25 cm. The individual plants were subjected to routine field investigations and fiber quality measurements. The fiber length of 604 individual ...

Embodiment 2

[0060] Example 2: Molecular marker selection method for improving fiber length traits of upland cotton

[0061] Using the molecular marker SSRN106 obtained in Example 1 270 and SSRN189 240 Molecular marker selection is carried out in breeding populations related to Sea Island Cotton Sea 1, MBI7747, etc., including the following steps:

[0062] (1) DNA extraction: take Haidao Mianhai 1 as the donor parent, and upland cotton varieties or strains (such as Zhongmian Institute 60, Lumianyan 28, Xinluzao 50, Ji 08) as the recipient parent, carry out hybridization, return Cross to obtain segregated populations, or use Haidao Mianhai 1 as the donor parent and upland cotton varieties (such as Zhongmian 60, Lumianyan 28, Xinluzao 50, Ji 08) as recipient parents to obtain high-generation backcross The substituent line and its derivatives, or the progeny of the hybridization and backcrossing of the substitution line MBI7747 and the upland cotton variety, the DNA of a single plant of the...

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Abstract

The invention relates to the technical field of molecular breeding and specifically relates to molecular marker which is from sea island cotton 1 and related to fiber length and application thereof. The molecular marker is SSRN106270 and SSRN189240. The molecular marker is favorable for overcoming the defect of an existing breeding technology for identifying the fiber length, can improve fiber length selection efficiency and can quicken a high-quality novel variety breeding process.

Description

technical field [0001] The invention relates to the technical field of cotton molecular breeding, in particular to a molecular marker related to fiber length from Sea Island Cotton Sea 1 and its application. Background technique [0002] Cotton is an important economic crop in the world, and cotton fiber is an important raw material for textile industry. Cotton occupies an important position in my country's national economy. With the rapid development of the textile industry and the continuous improvement of people's living standards, the requirements for the quality of cotton fibers are also getting higher and higher. Upland cotton varieties are planted in a large area in my country. Before the 1980s, cotton breeders mainly focused on improving the yield, but did not pay enough attention to the improvement of fiber quality. As a result, most of the cotton varieties in my country showed high yield and high quality. Difference. In the middle and late 1990s, fiber quality bre...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13
Inventor 石玉真卢全伟袁友禄肖向辉刘爱英李俊文龚举武巩万奎葛群商海红潘境涛
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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