A kind of SNP site related to the rapid growth of largemouth bass and its identification method and application

A largemouth bass, a rapid technology, applied in the field of SNP markers and identification, can solve the problems of large growth disparity, mutual cannibalism, and unsatisfactory quality of largemouth bass parents, and achieves fast growth rate, guaranteed reliability, and reduced blindness effect

Active Publication Date: 2016-06-08
佛山市三水白金水产种苗有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method is convenient, fast, and simple, but it is greatly affected by human factors. In addition, largemouth bass is a carnivorous fish with strong predatory nature. If the bait is insufficient, it will eat each other, resulting in a large difference in growth.
It can be seen that judging the parental quality of largemouth bass only from phenotype often does not achieve the desired effect

Method used

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  • A kind of SNP site related to the rapid growth of largemouth bass and its identification method and application
  • A kind of SNP site related to the rapid growth of largemouth bass and its identification method and application
  • A kind of SNP site related to the rapid growth of largemouth bass and its identification method and application

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Embodiment

[0030] The applicant's research found that a SNP was found at the C+598G position of the largemouth bass cathepsin B (CTSB) sequence, which changed the amino acid from glycine (Gly) to arginine (Arg), and was typed by the Snapshot method Allele G and allele C were found to exist, constituting three genotypes GG, GC and CC. The sequence of the allele G is shown in SEQ ID NO: 1, and the C / G mutation of the 234th bp of the sequence shown in SEQ ID NO: 1, when it is a G base, the allele G is obtained.

[0031] The random population M with 165 samples used for correlation analysis in this experiment was bred in the same batch and reared in the same pond, and the sampling time was the same, so the differences in time, environment and artificial breeding conditions were not considered when establishing the model. The results of multiple comparisons of growth traits among individuals with different genotypes are shown in Table 2. The results of association analysis show that the genot...

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Abstract

The invention discloses a SNP site related to the rapid growth of largemouth bass, an identification method and application thereof. The SNP site is located at the 234th base of the largemouth bass cathepsin B gene sequence. The alleles of the SNP site are C and G, and there are three genotypes of CC, CG and GG. The largemouth bass The sequence of perch cathepsin B gene is shown in SEQ ID NO.1. The SNP site can be applied to the screening of fast-growing largemouth bass parents.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a SNP marker related to the rapid growth of largemouth bass, an identification method and application thereof. Background technique [0002] Largemouth bass (Micropterus salmoides L.), commonly known as California perch, is native to North America. It has the characteristics of fast growth, low temperature resistance, delicious meat and easy fishing. It is one of the important freshwater cultured fish. Introduced from Taiwan to Guangdong Province in 1983, it is now cultivated in most parts of the country. However, at present, the largemouth bass cultured in my country is domesticated from wild species, lacks directional selection, and often selects small individuals and early sexually mature individuals as parents when the parents are kept, resulting in the germplasm of largemouth bass in my country. The decline is mainly manifested in the decrease of growth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 李胜杰白俊杰周春龙樊佳佳马冬梅全迎春姜鹏于凌云
Owner 佛山市三水白金水产种苗有限公司
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