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Composition containing ohioensins f as a polytrichastrum alpinum-derived novel compound for preventing or treating arteriosclerosis

A technology of arteriosclerosis and composition, applied in the field of compositions for preventing or treating inflammatory diseases, to achieve the effect of treating and preventing arteriosclerosis or inflammatory diseases

Inactive Publication Date: 2014-09-24
KOREA INST OF OCEAN SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] For this reason, Korean Patent No. 0957205 discloses a benzonaphthoxanthenone-based compound bloxanthenone F isolated from Physcomitrella blondii that inhibits protein tyrosine phosphatase 1B as an active ingredient. A pharmaceutical composition for lowering blood sugar, however no studies have been conducted on the Physcomitrella blondii extract for modulating the expression of cell adhesion molecules

Method used

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  • Composition containing ohioensins f as a polytrichastrum alpinum-derived novel compound for preventing or treating arteriosclerosis
  • Composition containing ohioensins f as a polytrichastrum alpinum-derived novel compound for preventing or treating arteriosclerosis
  • Composition containing ohioensins f as a polytrichastrum alpinum-derived novel compound for preventing or treating arteriosclerosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Embodiment 1: Preparation of pseudoblond moss (Polytrichastrum alpinum) extract

[0068] Pseudoblond moss (Polytrichastrum alpinum (Hedw.) G.L.Sm.) was personally collected in Sejong base (S62 ° 13.3) of Antarctic King George Island (King George Island) in January, 2003 by one of the inventors of the present invention (J.H.Yim). ',W58°47.0') on the Barton Peninsula (Barton Peninsular).

[0069] The collected dry sample (50 g) of Polytrichastrum alpinum (Hedw.) G.L.Sm. was extracted with methanol (1 L×2) for 24 hours, then concentrated under reduced pressure to obtain 4.7 g of crude MeOHextract.

Embodiment 2

[0070] Example 2: Isolation of the benzonaphthoxanthone-series compound philodendron F from Pseudomonas blondii

[0071] After loading 4.7 g of the crude methanol extract of Physcomitrella blondii prepared in Example 1 on C18 functionalized silica gel flash column chromatography (C18functionalized silica gel flash column chromatography, 3x15cm, Aldrich), the 4.7 g of the crude extract of Physcomitrella blondii prepared in the above-mentioned example 1 was sequentially mixed in every 400 mL of water. 20%, 40%, 60%, 70%, 80%, 90%, and 100% (v / v) methanol mixed solvents were respectively injected to obtain fractionated products. Then inject the fractionated product eluted with 80% aqueous methanol into a semi-preparative reverse-phase (semi-preparative reverse-phase) high-performance liquid chromatography (HPLC), and for water (0.1% formic acid (formic acid) ) using a mixed solvent of acetonitrile (CH3CN) at a concentration of 40 to 60% for about 27 minutes to isolate the compoun...

experiment example 1

[0072] Experimental Example 1: Cell Culture

[0073] The vascular smooth muscle cell line MOVAS-1 was purchased from the American Standard Biological Collection (ATCC) (Rockville, MD), and was prepared in the presence of 200 mg / ml G418 (a kind of aminoglycoside antibiotics), 100 IU / ml penicillin, and 100 mg / ml chain Mycin and 10% fetal bovine serum (FBS) (Carlsbad, CA) in DMEM medium (Carlsbad, CA) were maintained at 37°C and 5% CO2. In the case of subculture, the cells were divided with 0.125% trypsin containing 0.01M ethylenediaminetetraacetic acid (EDTA). The cells used in this experiment were subcultured from the first to the sixth time. All All experiments were carried out with the same configuration of MOVAS-1 in a single donor. Mononuclear macrophages (THP-1) (ATCC), a human myelomonocytic cell line, were used for the cell adhesion assay of MOVAS-1. These cells were cultured in RPMI1640 (one type of medium) containing 2 mM L-glutamine, 100 µg / ml streptomycin, 100 IU / m...

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Abstract

The present invention relates to a composition containing extracts of polytrichastrum alpinum for preventing or treating arteriosclerosis. More particularly, the present invention relates to a pharmaceutical composition containing, as an active ingredient, ohioensins F which is a benzonaphthoxanthenone-based compound separated from Antarctic lichens polytrichastrum alpinum, for preventing or treating arteriosclerosis. The composition containing ohioensins F which is a benzonaphthoxanthenone-based compound derived from polytrichastrum alpinum according to the present invention may inhibit an expression of cell adhesion molecules induced by TNF-alpha, and therefore, can be valuably used in treating and preventing arteriosclerosis.

Description

technical field [0001] The present invention relates to a composition for preventing or treating arteriosclerosis containing a new compound blondsin F derived from an extract of Phrysus blondii, and a composition containing a new compound blondsin F derived from A composition for preventing or treating inflammatory diseases. Background technique [0002] Atherosclerosis is a disease of blood vessels in which viscous fluid containing fat deposits on the surface of the inner walls of arteries, blocking blood flow to the tissue, causing tissue death and, if the tissue is the brain or heart muscle, a stroke and cardiac palsy. Arteriosclerosis is not a single disease induced by a specific reason, but is induced by a combination of factors such as age, gender, genetic environment, mental stress, obesity, smoking, drinking, intake of unsaturated fat, diabetes, weight loss, etc. disease. [0003] In lesions of arteriosclerosis, specific cellular and molecular reactions will occur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61K31/35A61P9/10A61P9/00
CPCA23L1/3002A61K31/352A61K36/062A23V2002/00C07D311/78A61K9/146A23L33/105A61P9/00A61P9/10A23V2200/326A23V2250/21A61K31/35
Inventor 任挺汉金一赞金德圭韩世锺李亨锡吴贤哲表晳能
Owner KOREA INST OF OCEAN SCI & TECH
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