A Method for Degrading Straw Lignin by Cerexonas cerevisiae

Abstract

The invention provides a method for degrading straw lignin by using Ceriporiopsis subvermispora, which belongs to the field of bioengineering. According to the method, Ceriporiopsis subvermispora is used as an original strain and straw lignin is degraded through solid state fermentation. Production process flow of the method comprises the following steps: pretreatment procedures consisting of crushing, pre-wetting, etc.; liquid shake-flask culture of Ceriporiopsis subvermispora; primary seed culture; mixing with straw and solid state fermentation; and degradation of lignin in the straw. The fermentation technology is simple and convenient, poses no pollution to the environment and wastes no resource. With the method, the degradation rate of lignin is 70 to 96%, and straw with degraded lignin can be used for preparation of fuel ethanol.

Description

technical field [0001] The invention relates to the field of bioengineering, and in particular refers to a process for degrading lignin in straws by the fungus Acetophagous cerevisiae. Background technique [0002] Energy is the driving force for the survival and sustainable development of human society. At present, 80% of the energy used in the world is non-renewable fossil fuels. However, these fuel resources are limited and will be exhausted sooner or later. In addition, human beings use fossil fuels. Emit large amounts of carbon dioxide and sulfur dioxide gases into the atmosphere, causing global warming. Biomass energy is a substitute for fossil energy, and it has the advantages that fossil energy cannot replace, that is, biomass energy is clean energy, and resources are not limited. Therefore, the development of biomass energy is a key measure to solve the energy crisis. [0003] my country is a large agricultural country, with an annual output of more than 600 millio...

AI Technical Summary

Problems solved by technology

Steam explosion is to use high-pressure steam to infiltrate the interior of the fiber and release it from the closed space in the form of air flow. The fiber undergoes a certain mechanical fracture, and most of the hemicellulose and a small amount of lignin and cellulose in the raw material are degraded and dissolved. The composition of the method is relatively high, large-scale equipment is required, and lignin needs to be treated; the acid treatment method uses sulfuric acid to hydrolyze glycosidic bonds, ester bonds, etc., so that the network structure of corn stalk fibers is loose, but this method consumes a large amount of organic acid or Inorganic acid and lignin still need to be further processed and are not suitable for large-scale industrial production

Alkali treatment and ammonia water treatment use lignin to dissolve in alkaline solution, and there are also environmental pollution problems like acid treatment

This is due to the fact that these methods generally have problems such as difficult control of reaction conditions, high reaction temperature, long treatment time, large space occupation, unsatisfactory treatment effect, and inability to remove lignocellulose.

Images