Uniform mixing device for nucleic acid extraction

Abstract

The invention discloses a uniform mixing device for nucleic acid extraction. The uniform mixing device comprises a mounting rack, a driving motor, a driving gear, multiple driven gears, and a magnetic bar sleeve which corresponds to the multiple driven gears one by one, wherein a body part of the driving motor is fixed on the mounting rack; the multiple driven gears and the driving gear are pivoted on the mounting rack and are arranged along the line; the driving gear, a driven gear adjacent to the driving gear, and adjacent two driven gears are matched with one another in a meshed mode; the driving gear is synchronously coupled to a rotating shaft of the driving motor; the magnetic bar sleeve is arranged below the mounting rack, and at least one stirring blade is arranged at the outer periphery of the magnetic bar sleeve; the top of the magnetic bar sleeve is synchronously coupled to the corresponding driven gears. The liquid can be prevented from splashing from the top of a test tube, and an aerosol phenomenon is reduced, so that cross infection is effectively reduced, and the sample detection accuracy is improved. Meanwhile, the uniform mixing efficiency is high, the uniform mixing device can be matched with a magnetic bead separation device, and rapid nucleic acid separation is realized.

Description

technical field [0001] The invention relates to a mixing device for nucleic acid extraction. Background technique [0002] In the fields of disease control, food safety, forensic identification, genomics, etc., it is usually necessary to extract biological active substances (such as DNA) as the object of detection and research; the extraction of traditional biological active substances usually includes cracking, purification, For washing and other processes, it is usually necessary to mix the added reagent with the sample in the purification process. For example, when using the magnetic bead separation method for purification, after adding the reagent with magnetic beads to the sample, it is necessary to use a pipette to insert Into the sample test tube, use the vacuum pump to control the repeated suction of the tip of the pipette, that is, the so-called pipette sample, so that the reagent and the sample are fully mixed. The mixing device of this structure is used in actual ...

AI Technical Summary

Problems solved by technology

[0002] In the fields of disease control, food safety, forensic identification, genomics, etc., it is usually necessary to extract biological active substances (such as DNA) as the object of detection and research; the extraction of traditional biological active substances usually includes cracking, purification, For washing and other processes, it is usually necessary to mix the added reagent with the sample in the purification process. For example, when using the magnetic bead separation method for purification, after adding the reagent with magnetic beads to the sample, it is necessary to use a pipette to insert Into the sample test tube, use the vacuum pump to control the repeated suction of the tip of the pipette, that is, the so-called pipette sample, so that the reagent and the sample are fully mixed. The mixing device of this structure is used in actual use. , because it is difficult to control the suction force of the vacuum pump, it is easy to cause the splash of the sample when sucking the sample, resulting in cross-infection between the samples, and at the same time, aerosol will be generated inside the sample, which will affect the accuracy of the sample detection

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