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Cicada slough protease enzymolytic product and application thereof

A technology of protease hydrolysate and enzymolysate, applied in the field of traditional Chinese medicine pharmacy, can solve the problems of microbial contamination, unclear active ingredients, large dosage, etc.

Inactive Publication Date: 2014-09-24
HEBEI YILING MEDICINE INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main components are chitin, protein, amino acid, etc. The active ingredients in the medicinal materials are not clear, so at present, the whole powder is used as medicine, which has the disadvantages of large dosage and easy to cause microbial contamination.

Method used

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  • Cicada slough protease enzymolytic product and application thereof
  • Cicada slough protease enzymolytic product and application thereof
  • Cicada slough protease enzymolytic product and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] (1) Net select 8g of cicada slough, and crush it into ultra-fine powder.

[0091] (2) Put the superfine powder of cicada slough into a 250ml round bottom flask, add artificial gastric juice according to the solid-liquid ratio of 1:20, seal it, and act in a constant temperature water bath at 37°C for 30 minutes, then add 3% pepsin for hydrolysis for 3 hours to obtain pepsin enzyme For the hydrolyzate, the pepsin hydrolyzate was placed in a constant temperature water bath at 85°C for 15 minutes to inactivate the enzyme, cooled to room temperature, and centrifuged at 4500 r / min for 15 minutes, and the obtained supernatant and residue were used for later use.

[0092] (3) Take the supernatant and dry it to obtain the cicada slough pepsin hydrolyzate.

[0093] (4) Take the residue and add artificial intestinal juice at a solid-to-liquid ratio of 1:20, seal it tightly, and put it in a constant temperature water bath at 45°C for 30 minutes, then add 5% trypsin to hydrolyze it ...

Embodiment 2

[0096] (1) Net select 5g of cicada slough, and crush it into ultra-fine powder.

[0097] (2) Put the superfine powder of cicada slough into a 250ml round bottom flask, add artificial gastric juice according to the solid-liquid ratio of 1:20, seal it, and act in a constant temperature water bath at 37°C for 30 minutes, then add 3% pepsin for hydrolysis for 3 hours to obtain pepsin enzyme For the hydrolyzate, the pepsin hydrolyzate was placed in a constant temperature water bath at 85°C for 15 minutes to inactivate the enzyme, cooled to room temperature, and centrifuged at 4500 r / min for 15 minutes, and the obtained supernatant and residue were used for later use.

[0098] (3) Take the supernatant and dry it to obtain the cicada slough pepsin hydrolyzate.

[0099] (4) Take the residue and add artificial intestinal juice at a solid-to-liquid ratio of 1:20, seal it tightly, and put it in a constant temperature water bath at 45°C for 30 minutes, then add 5% trypsin to hydrolyze i...

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Abstract

The invention discloses cicada slough protease enzymolytic products and an application thereof. The cicada slough protease enzymolytic products are obtained by the following steps particularly: A, selecting cicada slough, crushing into fine powder; B, putting the cicada slough fine powder in artificial gastric juice, adding pepsase for hydrolysis to obtain a pepsase enzymolytic liquid, then performing centrifugation to obtain an supernatant and residues to keep in reserve; C, taking the supernatant, drying to obtain a cicada slough pepsase enzymolytic product; D, taking the residues, transferring the residues into artificial intestinal juice, adding trypsin for hydrolysis to obtain a cicada slough trypsin enzymolytic product. The pepsase enzymolytic product obtained in step C and the trypsin enzymolytic product obtained in step D are the cicada slough protease enzymolytic products. The cicada slough protease enzymolytic products have anticoagulation or thrombolytic effect.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine pharmacy, in particular to cicada ecdysis protease hydrolyzate and application thereof. Background technique [0002] The husk shed by a nymph of the Cicadae Cryptotympana pustulata Fabricius as it emerges. Sweet in nature, cold in taste, returns to lung and liver channels. It has the functions of evacuating wind-heat, relieving sore throat, clearing rash, improving eyesight and removing nebula, and relieving spasm. It is mainly used clinically for wind-heat cold, sore throat, hoarseness, impermeable measles, rubella itching, red eyes and nebula, convulsions, convulsions, Tetanus etc. The main components are chitin, protein, amino acid, etc. The active ingredients in medicinal materials are not clear, so at present, most of them are used as whole powder, which has the disadvantages of large dosage and easy to cause microbial contamination. In view of the fact that animal proteins, mu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/64A61K38/01A61P7/02
Inventor 李晓燕赵韶华王鑫国王玉蓉
Owner HEBEI YILING MEDICINE INST
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