Cordyceps militaris strain cultivation method
A technology for culturing Cordyceps militaris and strains, applied in the field of cultivating Cordyceps militaris strains, can solve the problems of poor stability, easy degradation and aging of the parent species, and achieve the effects of reducing prices and increasing yields
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[0049] The culture method of Cordyceps militaris bacterial classification comprises the following steps:
[0050] 1. Mother seed preparation
[0051] The mother seed preparation adopts the spore separation method, which belongs to the sexual reproduction method. It uses the principle of self-ejecting spores from fungal fruiting bodies under sterile conditions, so that spores are scattered on the artificial medium, and the original bacterial system is obtained through cultivation, and then transferred into inclined test tubes for cultivation. Specifically include the following steps:
[0052] (1) Production medium
[0053] The culture medium can choose the following two formulas for production:
[0054] 1. Glucose 20 grams, agar 20 grams, peeled potatoes 200 grams (boiled juice), water 1000 mg (PDA medium)
[0055] 2. 30 grams of oatmeal, 20 grams of agar, 1000 ml of water
[0056] Preparation method: add water to oatmeal, heat in a boiling water bath for 1 hour, filter wi...
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