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Multiplex Amplification Kit for 20 Y Chromosome Short Tandem Repeats

A composite amplification, primer sequence technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of insufficient effectiveness, inability to use different parts, waste of DNA database data resources, etc. Achieve the effect of high compatibility and high individual recognition rate

Active Publication Date: 2017-01-18
BEIJING PEOPLESPOT TECH
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AI Technical Summary

Problems solved by technology

In this way, not all data are valid when comparing, but only the data of the same loci can be compared, while different parts cannot be applied
Therefore, due to the different loci of each STR kit, some data resources of the DNA database are wasted, and the effectiveness is not enough

Method used

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  • Multiplex Amplification Kit for 20 Y Chromosome Short Tandem Repeats
  • Multiplex Amplification Kit for 20 Y Chromosome Short Tandem Repeats
  • Multiplex Amplification Kit for 20 Y Chromosome Short Tandem Repeats

Examples

Experimental program
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Effect test

Embodiment 1

[0047] Example 1 multiplex amplification of 20 Y-STR gene loci and analysis of their genotypes

[0048] Purchase 9948 cell line. Template DNA was extracted from the cell line using the chelex-100 method. The amplification reaction was carried out on the ABI9700 thermal cycler, the electrophoresis and detection were carried out on the ABI3100 genetic analyzer, and the data analysis was performed using GeneMapper ID v3.2 software. The samples were tested with AmpFISTR Yfiler kit from ABI company and AGCU Y18 kit from Zhongde Meilian company. The operation method was carried out according to the kit instructions, and the results were used as a control. The reagents and materials used in the present invention, such as allelic ladder (ladder), are conventional materials commonly used by those skilled in the art.

[0049] 1.1. chelex-100 method to extract DNA (specific method refer to "Forensic DNA Protocol", HumanaPress, 1998)

[0050] 1) Take 3 μl of cell lines in a 500 μl cent...

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Abstract

The invention relates to a composite amplification system used for simultaneous analysis of multiple Y-STR loci. The system is characterized in that the system can realize composite amplification of 20 Y-chromosome loci consisting of DYS19, DYS388, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385a, DYS385b, DYS437, DYS438, DYS439, DYS447, DYS448, DYS456, DYS458, DYS635, DYS460 and Y-GATA-H4. The invention also relates to a method and a kit for simultaneous analysis of DNA samples and application of the method and the kit.

Description

[0001] Technical field: [0002] The present invention relates to the field of biotechnology. Further, the present invention relates to the detection of polymorphic genetic marker genes in the human genome. In particular, the present invention relates to the simultaneous amplification of multiple short tandem repeat loci in one amplification system using polymerase chain reaction. [0003] Background technique: [0004] Short tandem repeats (STR for short), also known as microsatellites or simple sequence repeats (SSR), are a type of DNA tandem repeats widely present in eukaryotic genomes, with a core sequence of 2-6 base repeat units. The number of STR loci is large and widely distributed, accounting for about 3% of the entire genome (International Human Genome Sequencing Consortium, 2001), and the polymorphism is high. Species differences follow the Mendelian law of inheritance in the genetic process. Therefore, STR amplification detection technology is widely used in indi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 张威张琰
Owner BEIJING PEOPLESPOT TECH
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