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Acinetobacter calcoaceticus capable of efficiently degrading lignin

A technology of lignin and calcium acetate, applied in the direction of bacteria, microorganism-based methods, water/sludge/sewage treatment, etc., can solve the problem of difficult biodegradation of lignin

Inactive Publication Date: 2014-09-03
NORTHWEST A & F UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a strain of Acinetobacter calcoaceticus that can efficiently degrade lignin, so as to solve the bottleneck problem that lignin biodegradation is difficult

Method used

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  • Acinetobacter calcoaceticus capable of efficiently degrading lignin
  • Acinetobacter calcoaceticus capable of efficiently degrading lignin
  • Acinetobacter calcoaceticus capable of efficiently degrading lignin

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Effect test

Embodiment 1

[0024] Acinetobacter calcoaceticus LG-1 has been registered and preserved in China Center for Type Culture Collection (CCTCC); the preservation date is March 12, 2014; the number is CCTCC M 2014079.

[0025] The screening, identification and application procedures of the strain are as follows:

[0026] 1. Screening of bacterial strains

[0027] A: The sewage area of ​​the paper mill was sampled to obtain the sample sludge.

[0028] B: Prepare lignin inorganic salt culture solution and inorganic salt culture medium, and sterilize them in 100ml to 250ml Erlenmeyer flasks.

[0029] C: Configure 200g / L lignin mother liquor, sterilize it, and dialyze it with a sufficient amount of sterilized ddH2O (aseptic condition), and distribute it to a triangular flask containing 100mL inorganic salt medium to obtain Dialyze the lignin inorganic salt culture solution.

[0030] D: Prepare 0.9% (w / v) NaCl solution, sterilize it, add it to the sludge sample at a ratio of 1:100 (w / v), incubate ...

Embodiment 2

[0047] Embodiment 2: The degradation experiment of bacterial strain to lignin can also be verified by following experiments:

[0048] Streak the bacterial liquid obtained from culturing in LB medium on solid lignin inorganic salt medium and solid inorganic salt medium, and culture in a 37°C incubator for 12 hours to perform degradation verification.

[0049] The formula of described solid inorganic salt culture medium is: 1.55g K 2 HPO 4 , 0.85g NaH 2 PO 4 2H 2 0, 2.0g (NH 4 ) 2 SO 4 , 0.1g MgCl 2 6H20, 10mg EDTA, 2mg ZnSO 4 ·7H 2 0, 1mg CaCl 2 2H 2 0.5mg FeSO 4 ·7H 2 0,0.2 mg Na 2 MoO 4 2H 2 0,0.2mg CuSO 4 ·5H 2 0,0.4mg CoCl 2 ·6H 2 0, and 1mg MnCI 2 2H 2 0, Agar 15g;

[0050] The formula of the solid lignin inorganic salt medium using lignin as the sole carbon source is the same as that of the solid lignin inorganic salt medium, with 5g Lignin added.

[0051] The formula of the LB medium is: NaCl 10g, YEAST EXTRACT 5g, TRYPTONE 10g

[0052] The ex...

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Abstract

The invention provides acinetobacter calcoaceticus capable of efficiently degrading lignin. The acinetobacter calcoaceticus is characterized in that the acinetobacter calcoaceticus LG-1 is registered and collected in the China Center for Type Culture Collection (CCTCC) on March 12, 2014 with CCTCC No. M2014079. The acinetobacter calcoaceticus has a high-efficiency degradation effect on lignin and can grow in a lignin inorganic salt culture solution taking lignin as only carbon source, and the growing curve chart of the acinetobacter calcoaceticus shows that the growing effect of the acinetobacter calcoaceticus is obviously higher than that of rhodococcus serving as positive control and that of escherichia coli serving as negative control; the bacterial strain can be applied to the fields such as papermaking, energy sources and environmental protection; and the problem of great difficulty in degradation of alkali lignin in all industries is solved by using the high-efficiency degradation efficiency of the acinetobacter calcoaceticus for the alkali lignin.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of Acinetobacter calcoaceticus LG-1 capable of efficiently degrading lignin. Background technique [0002] Lignin accounts for about 30% of lignocellulose, and has great potential application value in the field of bioenergy and biomaterials. However, due to its complex structure, large molecular weight and no easily hydrolyzed units, lignin is recognized as one of the most difficult chemical and biological degradation of natural polymers. Papermaking wastewater is currently one of the most important sources of environmental pollution. Its main components are undegraded lignin compounds and their derivatives. These hard-to-degrade substances cause serious pollution to receiving water bodies, damage water ecosystems, and directly threaten human health. . In short, the difficult technical bottleneck of lignin degradation restricts the reuse of resources and also b...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01
Inventor 沈锡辉葛云辉刘应保司美茹刘翔韦林芳李梦琪李玉菲崔博宇王瑶
Owner NORTHWEST A & F UNIV
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