A kind of cultivation method of dry pinto bean tissue culture seedling

A cultivation method and technology for dried pinto beans, which are applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems that seedlings are susceptible to diseases and insect pests, limit the utilization of dried pinto beans as medicinal plants, and are difficult to mass-produce, and achieve the goal of overcoming the problems of seedlings. It is not easy to germinate, realizes mass production, and has the effect of high rooting rate

Inactive Publication Date: 2016-06-08
GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the collection of dried pinto bean is mainly wild. With the logging of people, the wild growing area of ​​dried pinto bean has been gradually reduced in recent years, which largely limits the utilization of dried pinto bean as a medicinal plant.
The existing artificial cultivation method of dried pinto bean mainly uses the seeds produced by domestication of wild plants for sowing. First, this method has problems such as that the seeds are not easy to germinate, and the seedlings are vulnerable to diseases and insect pests. Second, this method is difficult to produce in large quantities. Unable to meet the needs of modern large-scale planting

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  • A kind of cultivation method of dry pinto bean tissue culture seedling

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Experimental program
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Effect test

Embodiment 1

[0024] 1) In April, choose mature, bright in color, full, plump dried Pinto bean seeds and wash them with running water for 20 minutes, soak them in 70% (volume) ethanol for 20 seconds on the ultra-clean workbench, wash them with sterile water for 3 times, Concentration is 0.3% (mass) after immersing in the mercuric chloride of 8min, take out, inoculate on the aseptic sprout culture medium after washing 3 times with distilled water, in every 50ml Erlenmeyer flask, 5~8 seeds, after light culture 23d, Germinated dried pinto bean seedlings were obtained; wherein, the formula of the aseptic sprout culture medium was: 1 / 2MS+GA31.5mg / L+sucrose 30g / L+NAA2mg / L+agar 5g / L, the pH was 5.8; the light culture conditions were : light intensity 1500lx, light time 18h / d, temperature 26-28°C; dark treatment 6h / d, temperature 16-18°C, replace the medium every 6 days;

[0025] 2) Select disease-free and vigorously grown dried pinto bean seedlings, cut out the stems with leaves and place them in ...

Embodiment 2

[0031]1) Select a robust 5-year-old dried flower bean mother plant, and artificially pollinate it when it blooms. From October to December, harvest the dried flower bean pods that have been artificially pollinated and developed to maturity but have not yet cracked, and store them according to conventional methods ,spare;

[0032] 2) In April of the next year, choose the bright, substantial and plump dried Pinto bean seeds and wash them with running water for 20 minutes, soak them in 70% (volume) ethanol for 20 seconds on the ultra-clean workbench, wash them with sterile water for 3 times, and wash them with sterile water at a concentration of After soaking in 0.1% (mass) mercuric chloride for 10 minutes, take it out, rinse it with distilled water for 3 times, and inoculate it on the aseptic sprout culture medium, put 10 seeds in each 50ml triangular flask, and cultivate it under light for 25 days to obtain germinated dry flowers Bean seedlings; wherein, the formula of the ster...

Embodiment 3

[0039] 1) In April, select mature, bright in color, full, plump dried Pinto bean seeds to wash with running water for 30 minutes, soak them in 75% (volume) ethanol for 20 seconds on an ultra-clean workbench, wash them with sterile water for 3 times, Concentration is 0.1% (mass) after immersing in the mercuric chloride of 0.1% (mass) after soaking 10min, take out, inoculate on the aseptic sprout culture medium after washing with distilled water 3 times, in each 50ml Erlenmeyer flask, 8 seeds, after light culture 20d, obtain germination. dry pinto bean seedlings; wherein, the formula of the aseptic sprout culture medium is: 1 / 2MS+GA31.0mg / L+sucrose 25g / L+NAA3mg / L+agar 4g / L, pH is 6.0; light culture conditions are: light The intensity is 1800lx, the light time is 20h / d, and the temperature is 25-27°C; the dark treatment is 4h / d, the temperature is 17-19°C, and the medium is replaced every 5 days;

[0040] 2) Select disease-free and vigorously grown dried pinto bean seedlings, cut...

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Abstract

The invention discloses a cultivation method of fordia cauliflora hemsl tissue culture seedlings. The method comprises the following steps: (1) disinfecting fordia cauliflora hemsl seeds and inoculating in a sterile bud seedling culture medium, and carrying out illumination cultivation to obtain germinal fordia cauliflora hemsl seedlings; (2) selecting the germinal fordia cauliflora hemsl seedlings robust in growth and slicing into stems with axillary buds; (3) inoculating the sliced stems with axillary buds in an induction medium, and carrying out illumination cultivation till yellowish or milk white seedling tissues grow; (4) cutting the obtained seedling tissues into bulks and inoculating the bulks in a germination culture medium, and carrying out illumination cultivation to obtain fordia cauliflora hemsl cluster seedlings; and (5) dividing the obtained fordia cauliflora hemsl cluster seedlings, transplanting the fordia cauliflora hemsl cluster seedlings in a rooting culture medium, and carrying out illumination cultivation to obtain the fordia cauliflora hemsl rooting seedlings. By adopting reasonable culture media, the tissue culture seedling rooting rate is high. The whole method is simple to operate and can realize production on a large scale. The fordia cauliflora hemsl tissue culture seedlings obtained by cultivation are sterile and non-toxic and the survival rate after acclimatization and transplant is high.

Description

technical field [0001] The invention relates to plant tissue culture technology, in particular to a method for cultivating dried pinto bean tissue culture seedlings. Background technique [0002] Dried flower bean (Fordiacauliflora Hemsl) is a plant of the genus Dried flower bean of the Fabaceae Papilionaceae, also known as Yejingdou, Shuiluo umbrella, soil licorice, shrimp silk grass, Yulang umbrella and so on. The flowering period of dried pinto bean is from May to September, and the fruiting period is from June to November. The pods are club-shaped, flat, 7-10cm long, 2-2.5cm wide, leathery, truncate at the top, with a sharp beak, and gradually Narrow, flattened pilose, bald gradually, with 1-2 seeds; seeds round, flat, about 1cm wide, brown, smooth, caruncle membranous, wrapped in fungus stalk. Dried pineapple is pungent, flat, sweet, slightly sour, non-toxic, and has the effects of dispelling stasis and swelling, relieving pain and calming the mind, moistening the lung...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 刘金磊苏涛杨子明颜小捷王磊李典鹏
Owner GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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