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Methods Of Treatment Of Retinal Degeneration Diseases

A technology for retinal degeneration and disease treatment, applied in the field of retinal degeneration disease treatment

Inactive Publication Date: 2014-08-27
玛丽亚·皮娅·卡斯马 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, major issues include reducing the risk of viral integration and enabling the expression of oncogenes in order to generate pluripotent stem cells

Method used

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  • Methods Of Treatment Of Retinal Degeneration Diseases
  • Methods Of Treatment Of Retinal Degeneration Diseases
  • Methods Of Treatment Of Retinal Degeneration Diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0152]Example 2 shows that once the Wnt / β-catenin signaling pathway is activated, mouse retinal neurons can transiently reprogram in vivo back to the precursor stage following spontaneous fusion with transplanted cells such as HSPCs or ESCs . Newly formed hybrid cells reactivate neuronal precursor markers (eg HSPCs and ESCs reprogram retinal neurons back to Nanog and Nestin expression). Further, the hybrid cells can proliferate, differentiate along the neuro-ectodermal lineage (in the case of hybrid cells formed from HSPCs and retinal neurons), and finally enter terminally differentiated retinal neurons (such as photoreceptor cells), which Regenerates injured retinal tissue; alternatively, hybrid cells formed from ESCs and retinal neurons can proliferate and differentiate in endodermal and ectodermal lineages in addition to neuroectodermal lineages, which lead to teratoma form. Concomitant with retinal damage and induction of the Wnt / β-catenin signaling pathway in the eye, c...

Embodiment 1

[0208] Hematopoietic stem cell fusion triggers retinal regeneration in a mouse model of retinitis pigmentosa

[0209] 1. Method

[0210] cell preparation

[0211] Lineage-negative HSPCs were isolated from total bone marrow of Cre-RFP mice using Lineage Cell Depletion kits (Miltenyi Biotech) (mice stably expressing both CRE and red fluorescent protein [RFP]; courtesy of Jackson Laboratories ). They were treated with 1 μM BIO or PBS, and 1 μM tamoxifen for 24 hours before transplantation.

[0212] animal

[0213] R26Y rd1 Mice (mouse homozygous for the R26Lox-Stop-Lox-YFP transgene and rd1 mutation) [Srinivas et al., BMC Dev Biol 1, 4 (2001)].

[0214] transplant

[0215] will be 10 5 -10 6 Amounts of cells were transplanted into pre-anesthetized mice with intraperitoneal injection of ketamine: metomidine (mg / kg: 1.0 mg / kg, intraperitoneal injection), eyelids were carefully opened, under the ora serrata Make a small incision and inject 1 to 5 μl of a solution co...

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Abstract

The methods comprise administering cells having properties of stem cells or progenitor cells, to the retina and reprogramming of retinal cells mediated by cell fusion of said cells with said retinal cells, said reprogramming being mediated by activation of the Wnt / ss-catenin signalling pathway.

Description

technical field [0001] The present invention relates to the field of cell therapy or regenerative therapy for ophthalmic diseases. In particular, the present invention provides a method for treating retinal degenerative diseases by administering cells having properties of stem cells or progenitor cells to the retina and mediating cell fusion of the cells with the retinal cells to reprogram retinal cells, In the retinal cells such as retinal neurons or retinal glial cells, the reprogramming is mediated by activation of the Wnt / β-catenin signaling pathway. Background technique [0002] The retina is a specialized light-sensitive tissue at the back of the eye that contains photoreceptor cells (rods and cones) and neurons connected to a neural network for processing visual information. Rods function in low-light conditions, while cones are responsible for color vision and all those tasks that require high-resolution vision (such as reading). Rod cells are located primarily in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0793A61K35/28C12N5/0789C12N5/0735C12N5/0775A61K35/12A61K35/20
CPCC12N5/0606C12N5/0663A61K2035/124C12N5/0623A61K35/20C12N5/0647C12N2501/415A61K31/506A61K35/28A61P27/02A61P27/06A61P27/12
Inventor 玛丽亚·皮娅·卡斯马丹妮拉·桑格斯
Owner 玛丽亚·皮娅·卡斯马
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