Cross-linked sodium hyaluronate biomembrane and preparation method thereof

Abstract

The invention discloses a cross-linked sodium hyaluronate biomembrane and a preparation method thereof. The cross-linked sodium hyaluronate biomembrane is prepared by: conducting freeze drying on a prepared gel with a crosslinking degree up to 60%-80%, and then pressing the freeze-dried gel into a membrane. The preparation method of the gel includes: acquiring cross-linked sodium hyaluronate dry powder, then washing the collected sieved powder by dimethyl sulfoxide (DMSO); washing the DMSO washed powder by ethanol; then conducting vacuum drying to obtain cross-linked sodium hyaluronate powder; fully swelling the powder, performing purification for 6-10h at a room temperature of 15-35DEG C, then carrying out homogenization micronization treatment in a high-speed dispersion machine, and then collecting the uniform gel particles, thus obtaining the cross-linked sodium hyaluronate gel used for biomembrane preparation. The cross-linked sodium hyaluronate biomembrane prepared by the method provided by the invention has is pyrogen-free, sterile, free of residual crosslinking agent, and has low cytotoxicity. With long residence time in vivo, the biomembrane has good anti-adhesion effect, good biocompatibility and tissue repair performance.

Description

technical field [0001] The invention relates to a material used for surgical wounds, especially for preventing postoperative adhesions, in particular to a cross-linked sodium hyaluronate biofilm and a preparation method thereof. Background technique [0002] Clinically, there are high requirements for local hemostasis and postoperative anti-adhesion. Postoperative local bleeding and tissue adhesion may lead to serious clinical complications. Ideal hemostatic and anti-adhesion materials should have good biocompatibility, no obvious side effects, bioabsorbability, in vivo degradation rate synchronized with wound healing, rapid hemostasis, good tissue adhesion; and prevent postoperative adhesion effects Yes, it can promote the rapid healing of wounds and reduce mucosal damage. [0003] Among the anti-adhesion materials currently developed, the polylactic acid anti-adhesion film has low strength and poor flexibility. The maintenance time of collagen is short, mild inflammatory...

AI Technical Summary

Problems solved by technology

However, the low mechanical strength of the sponge makes it inconvenient to use, has no practical application value, and does not meet the requirements of industrialization.

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