Porcine pseudorabies virus gE IgM antibody colloidal gold immunochromatograohic assay test strip as well as preparation method and application thereof
A porcine pseudorabies virus and immunochromatographic detection technology, which is applied to measuring devices, analytical materials, biological material analysis, etc., can solve the problems of requiring special instruments and personnel, rarely detecting IgM antibodies, and long detection time, and achieves easy operation , The result is intuitive and easy to distinguish, and the effect is convenient to carry
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Embodiment 1
[0040] Schematic diagram of the structure of the colloidal gold immunochromatographic detection test paper card for porcine pseudorabies virus gE IgM antibody figure 1 As shown, the horizontal surface of the test strip in the test paper card is sequentially divided into water absorption area 1, solidified antibody area 2, gold standard probe area 3, and sample absorption area 4 from left to right. After the test strip is loaded into the card case 6, it is the porcine pseudorabies virus gE IgM antibody colloidal gold immunochromatography test card; water absorption area 1, solidified antibody area 2, gold standard probe area 3, sample absorption area 4, bottom plate 5 The materials are water-absorbing filter paper, nitrocellulose membrane, glass fiber membrane, polyester fiber membrane and polyethylene plate, and the cartridge 6 is a plastic cartridge with a viewing window and a sample hole. The detection line T 7 of the solidified antibody area 2 is coated with mouse anti-pig ...
Embodiment 2
[0058] Except that the amount of anti-pig IgM monoclonal antibody coated on detection line T 7 is 6 μg / cm; the amount of goat anti-mouse IgG coated on control line C 8 is 8 μg / cm; The labeling amount of the anti-porcine pseudorabies virus gE protein monoclonal antibody is 10 μg / mL, and the coating volume is 20 μL / cm; the method for colloidal gold-labeled anti-porcine pseudorabies virus gE protein monoclonal antibody: take the radius as 40nm pH value 8.2 Add 20 mL of colloidal gold with a concentration of 0.01%, add 200 μg of anti-porcine pseudorabies virus gE protein monoclonal antibody, combine it under stirring conditions, add BSA to a final concentration of 8%, and centrifuge to remove unbound anti-porcine pseudorabies virus gE protein monoclonal antibody and unstabilized colloidal gold particles and agglutinates, the deep red precipitate at the bottom of the centrifuge tube is dissolved with 2mL 0.01M pH 7.4 PBS buffer, which is the colloidal gold-labeled anti-porcine pseud...
Embodiment 3
[0060] The method of using the above-mentioned porcine pseudorabies virus gE IgM antibody colloidal gold immunochromatography test paper card:
[0061] Dilute 3-4 drops of the serum sample to be tested with 1mL 0.01M pH 7.4 PBS and drop 4-5 drops in the sample hole, and judge the result within 10-15 minutes. The red color of the control line C indicates that the test paper card is valid. The control line C and the detection line All Ts appear red, indicating that the sample PRV IgM antibody is positive, and only the control line C appears red, indicating that the sample PRV IgM antibody is negative.
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