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Polypeptides that regulate FGFR2 activity

An active, amino acid technology, applied in the field of peptides, to achieve the effect of small molecular weight, controllable quality and low immunogenicity

Active Publication Date: 2016-03-23
THE THIRD AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIV OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no peptide that specifically binds to FGFR2, so the development of peptides that can specifically bind to FGFR2 and regulate its activity has a good application prospect

Method used

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  • Polypeptides that regulate FGFR2 activity
  • Polypeptides that regulate FGFR2 activity
  • Polypeptides that regulate FGFR2 activity

Examples

Experimental program
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Embodiment Construction

[0015] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0016] The FGFR2 used in the preferred embodiment and the mouse anti-FGFR2 antibody were all purchased from Sigma Company of the United States, the phage 12 peptide library screening kit (including ER2738 strain) was purchased from New EnglandLab Company of the United States, and rabbits labeled with horseradish peroxidase (HRP) Anti-mouse IgG was purchased from Zhongshan Jinqiao Biological Reagent Co., Ltd., pcDNA3.1-Myc empty vector was preserved by our research group, Myc-FGFR2 plasmid was donated by Professor David Ornitz of Washington University, USA, high-efficiency eukaryotic transfection reagent VigoFect and dual luciferase reporter gene detection The kit was purchased from Weiglass Biotechnology (Beijing) Co., Ltd.

[0017] 1. Panning the peptides that can specifically bind to FGFR2 from the phage 12 peptide library

[0018] 1. Pa...

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PUM

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Abstract

The invention discloses a polypeptide capable of regulating the activity of FGFR2 (Fibroblast Growth Factor Receptor 2). The amino acid sequence of the polypeptide is Asn-Leu-Gly-Gln-Glu-Leu-Ala-Phe-Arg-Val-Pro-Asn. The polypeptide disclosed by the invention can be specifically combined with the FGFR2, plays an obviously regulating role on the activity of the FGFR2, can outstanding inhibit the activity of p-ERK (Phospho-Extracellular Signal-Regulated Kinase) contained in the main downstream signal channel MAPK (Mitogen Activated Protein Kinase) of the FGFR2, has the advantages of low molecular weight, easiness for preparation, controllability in quality, low immunogenicity and the like and has good potential application value in the field of medicines.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a polypeptide. Background technique [0002] FGFR (fibroblast growth factor receptor) is a kind of transmembrane tyrosine kinase receptor with autophosphorylation activity, combined with its corresponding ligand FGF (fibroblast growth factor), in the process of tissue and organ development and damage repair play an important role in. [0003] Four kinds of FGFRs have been found so far, namely FGFR1, FGFR2, FGFR3 and FGFR4. Among them, FGFR2 has an important function in bone and skull development. Previous studies have shown that two function-enhancing point mutations of FGFR2 (FGFR2-Ser252Trp, FGFR2-Pro253Arg) cause the most common human craniosynostosis—Apert syndrome, manifested as coronal suture closure, brachycephaly, and craniofacial dysplasia With severe syndactyly; combined with RNAi and Cre-loxP system to regulate the expression of FGFR2 gene, tamoxifen (tamoxifen) induced tr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08
Inventor 陈林谭乔燕金旻杜晓兰王晓凤王权徐伟
Owner THE THIRD AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIV OF PLA
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