Human colon cancer nude mouse skin subcutaneous transplantation tumor model establishing method

A nude mouse and model building technology for colorectal cancer, applied in medical science, veterinary instruments, veterinary surgery, etc., can solve the problem of single source of tumor cells

Inactive Publication Date: 2014-08-13
贵阳医学院附属医院
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Problems solved by technology

The disadvantage is that the source of tumor cells is single, which shows limitations in the study of individualization, such as the sensitivity of chemotherapy drugs, and cannot reflect the characteristics of individual changes in the biological

Method used

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  • Human colon cancer nude mouse skin subcutaneous transplantation tumor model establishing method
  • Human colon cancer nude mouse skin subcutaneous transplantation tumor model establishing method
  • Human colon cancer nude mouse skin subcutaneous transplantation tumor model establishing method

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Experimental program
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Embodiment

[0019] Step 1: Establishment of primary nude mouse xenograft tumor model

[0020] Fish-like non-necrotic tumor tissue at the edge of the tumor was excised from freshly isolated colorectal cancer specimens, rinsed repeatedly with normal saline, soaked in RPMI1640 solution containing double antibodies (500 U / ml each of penicillin and streptomycin), and put Put it in an ice box and send it to the laboratory quickly, take out the specimen on the ultra-clean workbench, put it in a petri dish, wash it repeatedly with PBS solution containing double antibodies, and cut off the fat and necrotic tissue outside the specimen; in the petri dish, use Ophthalmic scissors to cut tissue into 1mm 3 For the left and right small pieces, add 1-2 drops of RPMI1640 solution to the tissue during the cutting process to keep it moist; inject nude mice with pentobarbital sodium (70mg / kg) intraperitoneally, and use forceps to insert the tissue piece into the 20-gauge puncture needle, and then use the pu...

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Abstract

The invention provides a human colon cancer nude mouse skin subcutaneous transplantation tumor model establishing method. The method comprises the steps off cutting a tumor edge fish flesh shaped non-necrosis tumor tissue from a colon cancer specimen; shearing the tumor tissue into 0.8-1.3mm3 small blocks, performing primary transplantation and passage transplantation and finally studying individual specificity through a tumor model subjected to passage transplantation, such as the sensibility of a certain individual to chemotherapeutic drugs or radiotherapy.

Description

technical field [0001] The invention relates, in particular, to a method for establishing a subcutaneous transplanted tumor model of human colorectal cancer in nude mice. Background technique [0002] Colorectal cancer is a common tumor of the digestive tract with a high degree of malignancy and a serious threat to human health, and the incidence of colorectal cancer is increasing year by year. Therefore, the study of colorectal cancer has attracted the attention of the surgical community. In order to better study the biological characteristics of colorectal cancer, carcinogenesis mechanism, tumor diagnosis, anticancer drug screening, relationship between tumor and host, process of tumor invasion and metastasis, and effectiveness of treatment measures, a stable, reliable and reproducible Animal models are very necessary. The animal model has the risk of avoiding experiments on humans: it can strictly control the experimental conditions and increase the comparability of exp...

Claims

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Application Information

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IPC IPC(8): A61D7/00A61D1/00
Inventor 颜登国张东兴龚慧
Owner 贵阳医学院附属医院
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