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An elution method for liquid chromatography and inspection equipment thereof

An elution method and liquid chromatography technology, applied in analytical materials, measuring devices, instruments, etc., can solve the problems of increased instrument complexity, cross-contamination of eluent, and increased failure rate, and improve the reliability of equipment operation. , the effect of simplifying the structure and detection procedures, improving the accuracy and stability

Inactive Publication Date: 2014-08-06
SHENZHEN LIFOTRONIC TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, internationally recognized gold standard liquid chromatography is used clinically to detect glycosylated hemoglobin HbA1c. Among them, high performance liquid chromatography (HPLC) only uses two elution methods of ion concentration, that is, one high ion concentration elution method is used. Liquid and a low-concentration eluent; a mechanical six-way rotary distribution valve is installed in the machine to distribute eluents with different ion concentrations to elute different glycated hemoglobin components in the sample; due to the The ratio of eluents with different concentrations in the machine will increase the complexity of the instrument, increase the failure rate, serious cross-contamination of eluents, and high costs for the manufacture, maintenance, and use of the instrument.
And low-pressure liquid chromatography analysis method has the elution method that adopts two kinds of ion concentrations and four kinds of ion concentrations; Different glycosylated hemoglobin components of the sample are eluted by means of an electronic isolation valve to separately control an eluent with an ion concentration; the elution and separation effect of the two eluents is not complete, which directly affects the accuracy of the test results; and The separation effect of the four eluents is better than that of the two eluents, but the complexity of the instrument, the failure rate, the cost of manufacturing, maintenance and use of the instrument will increase accordingly

Method used

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  • An elution method for liquid chromatography and inspection equipment thereof
  • An elution method for liquid chromatography and inspection equipment thereof
  • An elution method for liquid chromatography and inspection equipment thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] exist figure 1 In the schematic diagram shown, three eluents with different ion concentrations are set, namely: A eluent 1, B eluent 2, and C eluent 3, and the outlet pipelines of the three eluent containers are respectively provided by Respective electronic isolation valves 14 are controlled, and under the action of the first peristaltic pump 4 and the second peristaltic pump 7, respectively, the resin 5 that has the sample to be tested can be adsorbed, enter the colorimetric cell 7 for chromatographic analysis after elution, and finally obtain The concentration of glycated hemoglobin.

[0032] exist figure 2 In the schematic diagram of the test equipment shown, the power supply 11 supplies power to the entire system. After the control device 8 detects the test start command issued by the operator, the sampling device 10 starts to sample, puts it in the mixing cup 17 for hemolysis, and then controls The device 8 issues an instruction to make the first peristaltic pu...

Embodiment 2

[0034] 1. Configure the corresponding eluent as shown in Table 1:

[0035] Table 1

[0036]

[0037] 2. The control device issues an instruction to control the sampling device to start sampling, put it in the mixing cup for hemolysis, then the control device issues an instruction to make the peristaltic pump run, and then the control device issues an instruction to the electronic isolation valve to switch samples and eluent A respectively , eluent B and eluent C, the control device monitors and controls the temperature (25°C) of the chromatography column in real time, when the sample passes through the chromatography column and enters the colorimetric cell, the photometer performs voltage sampling and returns to the control device .

Embodiment 3

[0039] 1. Configure the corresponding eluent as shown in Table 2:

[0040] Table 2

[0041]

[0042] 2. The control device sends an instruction to control the sampling device to start sampling, put it in the mixing cup for hemolysis, then the control device sends an instruction to make the peristaltic pump run, and then the control device sends an instruction to the electronic isolation valve to switch samples and eluent A respectively , eluent B and eluent C, the control device monitors and controls the temperature (25°C) of the chromatography column in real time, when the sample passes through the chromatography column and enters the colorimetric cell, the photometer performs voltage sampling and returns to the control device .

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Abstract

The invention provides an elution method for detection of glycosylated hemoglobin with liquid chramatography and inspection equipment thereof, the method including following steps: step (1): preparing eluents of three kinds of ion concentration; step (2): performing elution on ion exchange resin which contains a to-be-tested sample with the said eluents of three kinds of ion concentration; step (3): performing chromatographic analysis on the said liquid that undergoes elution, and determining the content of the elution component; step (4): determining the concentration of the glycosylated hemoglobin HbA1c. The method is capable of solving the problem of cross contamination caused in the detection of eluents of two kinds of ion concentration. Compared with the method using eluents of four kinds of ion concentration, the method simplifies the inspection procedure and the structure of the inspection equipment, improves the reliability of the operation of the equipment, reduces the cost of the equipment, and greatly improves the accuracy and stability of the inspection.

Description

technical field [0001] The invention relates to the field of liquid chromatography detection, in particular to an elution method for detecting glycosylated hemoglobin by low pressure liquid phase ion exchange chromatography and testing equipment thereof. Background technique [0002] The concentration of HbA1c, the most important subcomponent of glycosylated hemoglobin in human blood, directly reflects the average blood sugar level of diabetic patients for three months. This index is an important basis for clinical screening, diagnosis and treatment of diabetic patients. [0003] At present, internationally recognized gold standard liquid chromatography is used clinically to detect glycosylated hemoglobin HbA1c. Among them, high performance liquid chromatography (HPLC) only uses two elution methods of ion concentration, that is, one high ion concentration elution method is used. Liquid and a low-concentration eluent; a mechanical six-way rotary distribution valve is installe...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/74
Inventor 徐岩刘先成彭国庆胡文雍梅林祥
Owner SHENZHEN LIFOTRONIC TECH
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