Gene chip for integrated detection and on-substrate amplification method

Abstract

The invention discloses a gene chip for integrated detection and an on-substrate amplification method. The on-substrate amplification method comprises the following steps: (1) fixing specific nucleic acid primers on the substrate; (2) placing the substrate, on which the specific nucleic acid primers are fixed, in an amplification tube; (3) adding prepared a gene extract to be detected and an amplification system to the amplification tube; (4) placing the amplification tube in a gene amplification instrument for amplification; (5) after completion of the gene amplification, detecting fluorescent signals on the substrate so as to acquire genetic information. In the on-substrate amplification method, the general step of nucleic acid hybridization is removed, the benefits are greatly increased, and the cost is reduced; the specific nucleic acid primers are fixed on the substrate, and then the specific nucleic acid primers and the substrate are placed in the amplification tube for on-substrate PCRs (Polymerase Chain Reactions), so that the PCR reactions can be realized separately and the problem that primers interfere with one another during multiple PCRs can be solved; multiple pieces of genetic information can be detected integrally at the same time, and contamination, which can be generated in the amplification process and the product transfer process, is avoided; the used instrument is simple and universal, so that a common PCR instrument and a fluorescence microscope can complete the detection.

Description

technical field [0001] The invention relates to an on-chip PCR amplified gene chip, which can be used for integrated detection of gene information, so that corresponding functions such as gene amplification and detection can be completed in the same closed space without transfer operation. Background technique [0002] At present, polymerase chain reaction (PCR) is one of the indispensable technologies in various detection and research processes involving nucleic acid molecules such as molecular biology, gene detection, disease detection and diagnosis. The principle of PCR is the semi-conservative replication of DNA (deoxyribonucleic acid, Deoxyribonucleic acid). Double-stranded DNA can be denatured and unwound into single strands under the action of various enzymes. With the participation of DNA polymerase, according to the principle of complementary base pairing Replicate into two identical molecular copies. DNA can also be denatured and melted at high temperature, and ca...

AI Technical Summary

Problems solved by technology

[0003] However, this technology has the following problems: first, because of the high sensitivity of PCR amplification, cross-contamination is easy to occur in the process of amplification and transfer, and the contamination is easy to be amplified, and the phenomenon of "false positive" occurs, which not only affects the The judgment of the results often needs to be verified by other experiments.

However, it still has the step of DNA hybridization, for which it also specially designs and transforms the amplification tube.

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