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Preparation method and applications of glucose oxidase preparations

A technology of glucose oxidase and preparation, which is applied in the direction of biochemical equipment and methods, oxidoreductase, and microorganism-based methods, etc. It can solve the problems of uncertified feeding license, no finding, low ability to remove toxins, etc., and achieve the use method Simple, fast degradation and stable performance

Active Publication Date: 2014-08-06
河北省微生物研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The essence of the biological method is the action of enzymes. The characteristics of enzymes are natural, green, environmentally friendly, fast response, strong specificity, and no pollution. However, an ideal one that can effectively degrade aflatoxin B has not been found yet. 1 The main problems of enzyme preparation products are the low ability to remove toxins and the lack of certification of feeding permits.

Method used

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  • Preparation method and applications of glucose oxidase preparations
  • Preparation method and applications of glucose oxidase preparations

Examples

Experimental program
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Effect test

Embodiment 1

[0031] The preparation method of glucose oxidase preparation is to inoculate Penicillium notatum ACCC30443 in a fermenter containing a fermentation medium containing carbon source, nitrogen source, inorganic salt and trace elements for aerated fermentation to make a fermented liquid, and the fermented liquid is separated from solid and liquid , take the liquid as a glucose oxidase preparation, and store it in an environment of 2---10°C for later use. Fermentation medium is made up of the component of following mass percentage:

[0032] Sucrose 8%, sodium nitrate 0.7%, dipotassium hydrogen phosphate 0.03%, magnesium sulfate 0.06%, potassium chloride 0.06%, the balance is water, natural pH value;

[0033] The technological conditions of aerated fermentation are as follows:

[0034] The inoculum volume is seed volume: fermentation medium volume = 0.9:100 temperature 29°C, ventilation volume 1:0.9vvm, pressure 0.05Mpa, culture period not less than 60 hours, and 721 spectrophotome...

Embodiment 2

[0047] The difference between this embodiment and embodiment 1 is:

[0048] Fermentation medium is made up of the component of following mass percentage:

[0049] Sucrose 6%, sodium nitrate 0.5%, dipotassium hydrogen phosphate 0.01%, magnesium sulfate 0.04%, potassium chloride 0.04%, the balance is water, natural pH value;

[0050] The technological conditions of aerated fermentation are as follows:

[0051] The inoculum size is seed volume: fermentation medium volume = 0.7:100, temperature is 27°C, ventilation is 1:0.7vvm, pressure is 0.03Mpa, and the culture period is not less than 60 hours.

[0052] The culture medium used in the preparation of the slant strains is Chasporian culture medium, the temperature is 26° C., and the culture period is 5 days.

[0053] The preparation of described secondary liquid seed comprises the following process steps:

[0054] According to the volume of the primary seed: the volume ratio of the secondary seed medium=1.3:100, the primary see...

Embodiment 3

[0058] The difference between this embodiment and embodiment 1 is:

[0059] Fermentation medium is made up of the component of following mass percentage:

[0060] Sucrose 7%, sodium nitrate 0.6%, dipotassium hydrogen phosphate 0.02%, magnesium sulfate 0.05%, potassium chloride 0.05%, the balance is water, natural pH value;

[0061] The technological conditions of aerated fermentation are as follows:

[0062] The inoculum size is seed volume: fermentation medium volume = 0.8:100, temperature is 28°C, ventilation is 1:0.8vvm, pressure is 0.04Mpa, and the culture period is not less than 60 hours.

[0063] The culture medium used in the preparation of the slant strains is Chasporian culture medium, the temperature is 28° C., and the culture period is 4.5 days.

[0064] The preparation of described secondary liquid seed comprises the following process steps:

[0065] According to the volume of the primary seed: the volume ratio of the secondary seed medium=1.5:100, the primary s...

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Abstract

The invention belongs to preparation and applications of enzyme preparations, and particularly relates to a preparation method and applications of glucose oxidase preparations. Penicillium notatum ACCC30443 is inoculated to a fermentation tank of a carbon source, nitrogen source, inorganic salts and trace elements containing fermentation medium, through aerated fermentation, a fermented liquid is prepared, and then, the fermented liquid is subjected to solid-liquid separation, so that a glucose oxidase preparation is prepared. According to the invention, a problem that new pollutions and chemical residues easily exist in the prior art is solved; and the preparation method and applications of a glucose oxidase preparation disclosed by the invention have the advantages that a preparation process is simple, strains and raw materials are safe, and prepared glucose oxidase preparations are easy to use, rapid in aflatoxin B1 degradation speed, and high in degradation rate, and the like.

Description

technical field [0001] The invention belongs to the preparation and application of enzyme preparations, in particular to the preparation method and utilization of glucose oxidase preparations. Background technique [0002] Aflatoxin B 1 It is one of many mycotoxins, and it is also the most toxic one among the mycotoxins found so far. It is mainly produced by Aspergillus flavus and Aspergillus parasiticus. Its toxicity is 10 times that of potassium cyanide and 68 times that of arsenic. It has strong high temperature resistance (begins to decompose at 268°C). It widely exists in feed, and more than 90% of the feed is polluted by mycotoxins in the moldy rain season every year, and its aflatoxin B 1 The detection rate is as high as 80%. Aflatoxin B 1 After being fed by poultry and livestock, a large amount of it accumulates in the liver, causing liver enlargement and pathological changes, resulting in decreased liver function, hindering its normal function, lowering immunity...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/04A23L1/015A23K1/165C12R1/825A23K20/189A23L5/20
CPCC12N9/0006C12Y101/03004
Inventor 胡常英王云鹏秦艳梅马清河胡科峰秦慧娟蔡建成李军章淑艳刘丽娜范星韩韬李宾赵从波罗同阳高庆华郑翔
Owner 河北省微生物研究所有限公司
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