Myocardial infarction triple rapid detection kit and preparation method for same
A technology for detection kits and kits, applied in the field of biotechnology applications, can solve the problems of incomplete indicators, delayed warning time, complicated operation, etc., and achieve the rapid effect of kits
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Embodiment 1
[0045] Example 1. Preparation and detection of MPO-FABP3-CRP triple kit
[0046] 1 main material
[0047]1.1 MPO, FABP3 and CRP standard products: National Institute of Inspection and Quarantine; MPO, FABP3 and CRP specific paired antibody, goat anti-mouse IgG antibody: product of Beijing Shengqi Yanghuan Technology Co., Ltd.; chloroauric acid: product of Sigma Company; nitrocellulose (NC ) Membrane: SARTORIUS (Germany), CN140, product of Sartorius, Germany; bovine serum albumin (BSA), polyethylene glycol PEG20000, hydrolyzed casein: Sigma product; other commonly used reagents are analytical reagents.
[0048] 1.2 The clinical samples were obtained by the company in relevant hospitals, a total of 200 samples, including 100 serum samples, 50 plasma samples and 50 whole blood samples. The content distribution intervals of MPO, FABP3 and CRP were 6.25-400ng / ml and 3.125-200ng / ml respectively , 0.25-16 μg / ml.
[0049] 2 methods
[0050] 2.1 MPO antibody, FABP3 antibody and CRP ...
Embodiment 2
[0065] Example 2. Preparation of MPO-FABP3-cTnI Triple Kit
[0066] 1 main material
[0067] 1.1 MPO, FABP3 and cTnI standard products: National Institute of Inspection and Quarantine; MPO, FABP3 and cTnI specific paired antibody, goat anti-mouse IgG antibody: product of Beijing Shengqi Yanghuan Technology Co., Ltd.; chloroauric acid: product of Sigma Company; nitrocellulose (NC ) Membrane: SARTORIUS (Germany), CN140, product of Sartorius, Germany; bovine serum albumin (BSA), polyethylene glycol PEG20000, hydrolyzed casein: Sigma product; other commonly used reagents are analytical reagents.
[0068] 1.2 The clinical samples were obtained by the company in relevant hospitals, a total of 200 samples, 100 samples of serum, 50 samples of plasma and 50 samples of whole blood. Serum with a fixed value between 0.1 and 24.41ng / ml.
[0069] 2 methods
[0070] 2.1 MPO antibody, FABP3 antibody and cTnI antibody colloidal gold labeled trisodium citrate-tannic acid mixed reducing agent...
Embodiment 3
[0075] Example 3. MPO-FABP3-cTnI Triple Kit Serum Detection of Troponin Negative and Positive Patients
[0076] Randomly select 16 sera from patients with negative and positive troponin indicators, and proceed as follows:
[0077] 1) Equilibrate the detection reagent and sample to room temperature, take out the test paper card, and lay it flat;
[0078]2) Accurately draw 10 μl of serum sample, add it to a clean centrifuge tube, then dilute it 10 times with sample diluent (normal saline or PBS), and mix thoroughly;
[0079] 3) Use a pipette gun to draw 10 μl of the diluted sample and add it to the sample hole. Within 15 to 20 minutes, use a colloidal gold quantitative reader or a color card (half) to determine the quantitative results, see Table 1 below:
[0080] Table 1. MPO-FABP3-cTnI triple kit detection results of serum troponin negative and positive patients
[0081]
[0082]
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