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Blood purification material for removing rheumatoid factors, and preparation method thereof

A technology for rheumatoid factor and blood purification, applied in the field of biomedicine, can solve the problems such as the ability of action is difficult to effectively reflect, and achieve the effects of high IgM adsorption selectivity, good stability and simple structure

Active Publication Date: 2014-07-23
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For IgM whose Fc fragment is wrapped inside the molecule, it is difficult to effectively reflect the ability of 4-mercaptoethylpyridine

Method used

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  • Blood purification material for removing rheumatoid factors, and preparation method thereof
  • Blood purification material for removing rheumatoid factors, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043]Example 1: Preparation of 1-amino-3-(2-(4-pyridyl)-ethylmercapto)-2-propanol cross-linked agarose blood purification material with a coupling density of 1.4 mmol / g dry gel ( activated by carbonyldiimidazole)

[0044] The specific steps are the same as in Example 1, the difference is that: the amount of CDI is increased to 0.9g / 10mL wet glue, and the activation reaction is carried out at 20°C for 2h; when the ligand is coupled, the reaction is carried out at 25°C for 2h; when the remaining imidazole activation group is hydrolyzed, NaOH solution at pH 10 was used. Elemental analysis showed that the ligand coupling density was 1.4mmol / g dry glue.

Embodiment 2

[0045] Example 2: Preparation of 1-amino-3-(2-(4-pyridyl)-ethylmercapto)-2-propanol cross-linked agarose blood purification material with a coupling density of 2.03 mmol / g dry gel ( activated by carbonyldiimidazole)

[0046] The specific steps are the same as in Example 1, the difference is that the amount of CDI is increased to 1.1g / 10mL wet glue, and the activation reaction is carried out at 25°C for 1h; when coupling ligands, 1-amino-3-(2-(4-pyridyl )-Ethylmercapto)-2-propanol is used in an amount of 10 times the volume, and the reaction is carried out at 25°C for 3 hours; when hydrolyzing the remaining imidazole activation groups, a NaOH solution with a pH of 10 is used. Elemental analysis showed that the ligand coupling density was 2.03mmol / g dry glue.

Embodiment 3

[0047] Example 3: Preparation of 1-amino-3-(2-(4-pyridyl)-ethylmercapto)-2-propanol cross-linked agarose blood purification material with a coupling density of 2.61 mmol / g dry gel ( activated by carbonyldiimidazole)

[0048] The specific steps are the same as in Example 1, the difference is that the amount of CDI is increased to 1.2g / 10mL wet glue, and the activation reaction is carried out at 25°C for 1h; when coupling ligands, 1-amino-3-(2-(4-pyridyl )-Ethylmercapto)-2-propanol is used in an amount of 10 times the volume, and the reaction is carried out at 25°C for 3 hours; when hydrolyzing the remaining imidazole activating groups, a NaOH solution with a pH of 13 is used. Elemental analysis showed that the ligand coupling density was 2.61mmol / g dry glue.

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Abstract

The invention provides a blood purification material for removing rheumatoid factors, and a preparation method of the blood purification material, belonging to the technical field of biomedicine. The blood purification material comprises a solid phase carrier and a ligand fixed on the solid phase carrier by chemical coupling, wherein the solid phase carrier is a polysaccharide natural polymer material; the ligand is 1-amino-3-(2-(4-pyridyl)-ethyl sulfydryl)-2-propanol; the ligand fixed on the solid phase carrier by chemical coupling has the density of 1.4-2.8mmol / g dry solid phase carrier. The blood purification material is capable of selectively absorbing the rheumatoid factors in the blood, and is limited in nonspecific adsorption for blood plasma components such as human serum albumin, immunoglobulin G (Ig G) and the like; the blood purification material is low in preparation cost and stable in physicochemical property, and can be used as an absorption filler of a blood purification device for removing the rheumatoid factors in the blood of rheumatoid patients.

Description

technical field [0001] The invention relates to a blood purification material for removing rheumatoid factor and its preparation method and application, belonging to the technical field of biomedicine. Background technique [0002] Rheumatoid factors (RF) is an IgM anti-denatured IgG antibody, which has been proven to be closely related to the occurrence and development of a series of rheumatoid diseases, such as rheumatoid arthritis and systemic lupus erythematosus. For these diseases, reducing the concentration of rheumatoid factors in the patient's blood by means of in vitro clearance can significantly improve symptoms, and play a role in relieving and treating the disease (B.C.McLeod, Introduction to the Third Special Issue: Clinical Applications of Therapeutic Apheresis. J Clin Apheresis, 2000, 15(1-2):1-5), so the blood purification technology used to remove rheumatoid factor has been widely valued in clinical practice. [0003] Blood purification technology is a trea...

Claims

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Application Information

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IPC IPC(8): B01J20/24B01J20/30B01D15/08
Inventor 贾凌云任军
Owner DALIAN UNIV OF TECH
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