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A method for detection of active ingredients in Qishen Yiqi dripping pills

A technology of Qishen Yiqi dropping pills and a detection method, which is applied in the directions of measuring device, material separation, material analysis, etc., can solve the problems of high price, cumbersome operation steps, poor accuracy, etc.

Active Publication Date: 2016-08-31
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the production process, it is necessary to detect the intermediates and finished products of the preparation process of Qishen Yiqi Dropping Pills to control the quality of the product at any time. Some of the existing detection methods have poor accuracy and low sensitivity, and some use expensive reagents And instrument, some operation steps are loaded down with trivial details, in order to better control the quality of Qishen Yiqi dripping pills, the present invention develops a kind of detection method of active ingredient of Qishen Yiqi dripping pills

Method used

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  • A method for detection of active ingredients in Qishen Yiqi dripping pills
  • A method for detection of active ingredients in Qishen Yiqi dripping pills
  • A method for detection of active ingredients in Qishen Yiqi dripping pills

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Effect test

Embodiment 1

[0137] (1) The identification method of Panax notoginseng and Astragalus membranaceus, including the following steps:

[0138] ①Preparation of the test solution of Sanqi and Astragalus: Take 1.0g of Qishen Yiqi Dropping Pills, dissolve it with 5ml of ammonia-added test solution by ultrasound, and pass it through a D101 macroporous adsorption resin column (inner diameter 1cm, column length 5cm, flow rate 0.5~ 0.7ml / min); elute with 20ml of water, discard the eluent, then elute with 10ml of ether, and use the eluent for later use; continue to elute with 5ml of chloroform, discard the eluent, and then wash slowly with 4ml of methanol Remove, discard the initial eluent 1ml, and collect the 3ml methanol eluent as the test solution.

[0139] ②Preparation of Panax notoginseng and Astragalus reference substance solution: Notoginseng saponin R 1 Reference substance, ginsenoside Rg 1 Reference substance, astragaloside reference substance, made by adding methanol, containing notoginsen...

Embodiment 2

[0153] (1) The identification method of Panax notoginseng and Astragalus membranaceus, including the following steps:

[0154] ①Preparation of the test solution of Sanqi and Astragalus: Take 0.5g of Qishen Yiqi Dropping Pills, dissolve it with 3ml of ammonia-added test solution by ultrasound, and pass it through a D101 macroporous adsorption resin column (inner diameter 0.8cm, column length 7cm, flow rate 0.5 ~0.7ml / min); eluted with 18ml of water, discarded the eluent, then eluted with 8ml of diethyl ether, and used the eluent for later use; continued to elute with 3ml of chloroform, discarded the eluent, and then slowly eluted with 4ml of methanol For elution, discard the initial 2ml eluate, and collect the 2ml methanol eluate as the test solution.

[0155] ②Preparation of Panax notoginseng and Astragalus reference substance solution: Notoginseng saponin R 1 Reference substance, ginsenoside Rg 1 Reference substance, astragaloside reference substance, made by adding methano...

Embodiment 3

[0169] (1) The identification method of Panax notoginseng and Astragalus membranaceus, including the following steps:

[0170] ①Preparation of the test solution of Sanqi and Astragalus: Take 2g of Qishen Yiqi Dropping Pills, dissolve it by ultrasonication in 10ml of ammonia-added test solution, and pass it through a D101 macroporous adsorption resin column (inner diameter 1.2cm, column length 5cm, flow rate 0.5~ 0.7ml / min); elute with 25ml of water, discard the eluent, then elute with 15ml of ether, and use the eluent for later use; continue to elute with 10ml of chloroform, discard the eluent, and then wash slowly with 5ml of methanol Remove, discard the initial eluent 2ml, and collect the 3ml methanol eluent as the test solution.

[0171] ②Preparation of Panax notoginseng and Astragalus reference substance solution: Notoginseng saponin R 1 Reference substance, ginsenoside Rg 1 Reference substance, astragaloside reference substance, made by adding methanol, containing notog...

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Abstract

The invention provides a detection method for effective components of an astragalus Salvia Miltiorrhiza qi-benefiting dropping pill. The method includes identification of panax notoginseng, astragalus and Dalbergia odorifera and tanshinol content determination. Specifically, the method consists of: preparing a panax notoginseng and astragalus test solution, preparing a panax notoginseng and astragalus reference solution, conducting panax notoginseng and astragalus thin-layer identification, preparing a Dalbergia odorifera test solution, preparing a Dalbergia odorifera reference drug solution, performing Dalbergia odorifera thin-layer identification, and determining the tanshinol content by ultra-high performance liquid chromatography. Tests show that the method has very good linearity, repeatability, reproducibility and stability, as well as high recovery rate, and is conducive to more comprehensive control of the quality of the astragalus Salvia Miltiorrhiza qi-benefiting dropping pill.

Description

Technical field: [0001] The invention belongs to the field of detection of traditional Chinese medicines, and in particular relates to an identification method and a content determination method of Qishen Yiqi dripping pills. Background technique: [0002] Qishen Yiqi Dropping Pills (QSYQ) is a traditional Chinese medicine compound preparation composed of astragalus, salvia miltiorrhiza, notoginseng and balsamic oil. It was approved by the State Food and Drug Administration of China in 2003 for the treatment of coronary heart disease and angina pectoris. Stimulation, no obvious side effects. [0003] Preclinical pharmacodynamic tests showed that Qishen Yiqi Dropping Pills can reduce the infarction size of dogs with myocardial infarction, improve ischemic electrocardiogram, reduce serum enzyme CK and LDH activity, reduce plasma ET and TXB2 levels, and reduce 6-Keto-PGF1α Activity and 6-Keto-PGF1α / TXB2 ratio increase; it can reduce the infarct size and increase SOD activity ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90G01N30/02
Inventor 葛丹丹边宁侯春莲孙玉侠曹凤兰
Owner TIANJIN TASLY PHARMA CO LTD
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