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Extracting method of radix gypsophila saponin

A technology of carnation and extraction method, which is applied in the field of medicine, can solve the problems of toxicity, high energy consumption, and long time consumption, and achieve the effects of weak corrosion of equipment, simple and safe operation, and short preparation cycle

Inactive Publication Date: 2014-07-09
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional extraction of total saponins often uses the heat reflux method to process medicinal materials, but the heat reflux extraction method usually takes a long time and consumes a lot of energy; the traditional total saponin purification uses the n-butanol extraction method, but the dianthus medicinal material contains flavonoids, and n-butanol extraction cannot Effective separation of saponins and flavonoids leads to a decrease in the purity of total saponins; n-butanol itself has certain toxicity and is a flammable liquid, and there are potential safety hazards in a large number of applications

Method used

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  • Extracting method of radix gypsophila saponin
  • Extracting method of radix gypsophila saponin

Examples

Experimental program
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Effect test

Embodiment 1

[0030] ① 10g of Dianthus root is made into powder, and sieved with 80 mesh;

[0031] ②Extract with 50ml of 75% ethanol, ultrasonic for 60min, extraction times twice, temperature 60℃;

[0032] ③ Centrifuge at 2000 rpm for 10 min, and take the supernatant;

[0033] ④Dried at 80°C to form an extract, dissolved in 20ml of single distilled water, centrifuged at 2000 rpm for 10min, and took the supernatant;

[0034] ⑤ Extract 3 times with 20ml of petroleum ether, add 5% NaCl as demulsifier, and remove the lower aqueous phase;

[0035] ⑥ Pass the water phase through a D101 macroporous resin column, elute with 30% ethanol for 5 times, discard the lotion, elute with 75% ethanol for 5 times, and collect the middle 3 washes;

[0036] ⑦ The collected solution is freeze-dried to make a light brown powder, that is, total saponins;

[0037] ⑧Ultraviolet spectrophotometer 190-400nm wavelength scanning, found the absorption peak at 205nm, the total saponin content was 334.5mg.

Embodiment 2

[0039] ① 10g of Dianthus root is made into powder, and sieved with 80 mesh;

[0040] ②Extract with 70ml of 95% ethanol, ultrasonic for 40min, extraction times twice, temperature 60℃;

[0041] ③ Centrifuge at 2000 rpm for 10 min, and take the supernatant;

[0042] ④Dried at 80°C to form an extract, dissolved in 20ml of single distilled water, centrifuged at 2000 rpm for 10min, and took the supernatant;

[0043] ⑤ Extract 3 times with 20ml of petroleum ether, add 5% NaCl as demulsifier, and remove the lower aqueous phase;

[0044] ⑥Extract 3 times with 20ml of water-saturated n-butanol, and take the upper n-butanol phase;

[0045] ⑦The n-butanol phase was evaporated to dryness in a water bath in an evaporating dish to obtain a yellow-brown powder, namely total saponins;

[0046] ⑧Ultraviolet spectrophotometer 190-400nm wavelength scanning, found that there are absorption peaks at 190nm, 196nm, and 205nm, mainly at 205nm, and the total saponin content was 543.5mg.

Embodiment 3

[0048] ①Powder made from 500g of Dianthus root, and sieved with 80 mesh;

[0049] ②Extract with 2500ml of 75% ethanol, ultrasonic for 40min, extraction once, temperature 60℃;

[0050] ③ Centrifuge at 2000 rpm for 10 min, take the supernatant, and spin evaporate to recover ethanol;

[0051] ④ The concentrated solution was dried at 80°C to form an extract, dissolved in 500ml of single distilled water, centrifuged at 2000 rpm for 15min, and the supernatant was taken;

[0052] ⑤ Extract 3 times with 500ml of petroleum ether, add 10% NaCl as demulsifier, and remove the lower aqueous phase;

[0053] ⑥Water phase passed through D101 macroporous resin column, eluted with 30% ethanol for 5 times, discarded the lotion, eluted with 75% ethanol again for 5 times, collected 4 times of lotion;

[0054] ⑦ The collected solution is rotary evaporated to recover ethanol, and the concentrated solution is freeze-dried to make a light brown powder, namely total saponins;

[0055] ⑧Ultraviolet s...

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Abstract

The invention belongs to the field of medicines and particularly discloses an extracting method of radix gypsophila saponin. The radix gypsophila roots are taken as raw materials and grinded into powder, reflux extraction is replaced by ultrasonic extraction, multi-step extraction purification is replaced by macroporous resin purification, and a preparation process of the radix gypsophila saponin is optimized to obtain the best preparation condition, thereby facilitating industrial pharmaceutical production. The extracting method disclosed by the invention is simple and safe to operate, weak in equipment corrosion, short in preparation period, good in product purity and suitable for being widely popularized and applied.

Description

(1) Technical field [0001] The invention belongs to the field of medicine, in particular to a method for extracting dianthin. (2) Background technology [0002] Carnation ( Gypsophila oldhamiana Miq.) is the root of the herbaceous plant Stone Flower of the family Caryophyllaceae, also known as Xiacao, Ou Stone Flower, and Mountain Grasshopper. It is widely distributed throughout the country and is mainly produced in Shandong, Shanxi and other places. This plant is a commonly used herbal medicine in our country, and it is used as a substitute of Yinbupleurum in some areas, and it has been recorded in the "Dictionary of Chinese Medicine". The medicine is sweet and slightly cold in nature, and has the effects of clearing away heat and cooling blood, promoting blood circulation and dispelling blood stasis, reducing swelling and relieving pain, reducing putrefaction and promoting muscle growth. It mainly treats diseases such as consumptive muscle fever, bone steaming, chro...

Claims

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Application Information

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IPC IPC(8): A61K36/36
Inventor 谢靖战松梅韩彦弢张笛王春波
Owner QINGDAO UNIV
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