Fabrication of Nanofibrous Membrane Biosensors by Electrospinning

A biosensor, nanofiber membrane technology, applied in instruments, scientific instruments, material analysis by electromagnetic means, etc., can solve the problems of accuracy, sensitivity, operational stability, service life, insufficient selectivity, etc., and achieve high sensitivity , good reproducibility and high economic efficiency

Active Publication Date: 2016-08-31
扬州智创企业运营管理服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Before the present invention, existing methods for immobilizing bioactive molecules such as enzymes mainly included adsorption, cross-linking, covalent bonding, embedding, etc. There are deficiencies and limitations

Method used

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  • Fabrication of Nanofibrous Membrane Biosensors by Electrospinning
  • Fabrication of Nanofibrous Membrane Biosensors by Electrospinning
  • Fabrication of Nanofibrous Membrane Biosensors by Electrospinning

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Preparation of the hemoglobin enzyme sensor

[0033] The acrylonitrile-acrylic acid copolymer is prepared into a 6% N,N-dimethylformamide solution, and then a 8 mg / ml hemoglobin enzyme solution is prepared with a phosphate buffer solution, the polymer solution is used as the outer layer, and the enzyme solution is used as the inner layer. layer, then 15KV voltage, 15cm spinning distance, 40% humidity, and room temperature for electrospinning, the outer injection speed is set to 0.3mm / min, the inner injection speed is set to 0.1mm / min, spinning The time is set to 40 s, and a conventional glassy carbon electrode (0.3 mm in diameter) is used as a receiver to prepare a nanofiber membrane biosensor. With the electrode as the working electrode, the platinum wire electrode as the auxiliary electrode, and the saturated calomel electrode as the reference electrode, the biosensor has a constant potential of -0.3V and a phosphoric acid buffer solution with a pH of 7.0. 2 o 2 sol...

Embodiment 2

[0036] Preparation of polyphenol oxidase sensor

[0037] First prepare acrylonitrile-acrylic acid copolymer into N,N-dimethylformamide solution with a concentration of 6%, and then configure 8 mg / ml polyphenol oxidase solution with phosphate buffer solution. The polymer solution is used as the outer layer, enzyme The solution is used as the inner layer, and electrospinning is carried out at 15KV voltage, 15cm spinning distance, 40% humidity, and room temperature. The injection speed of the outer layer is set to 0.3mm / min, and the injection speed of the inner layer is set to 0.1m / min. , the spinning time was set to 40 s, and a conventional glassy carbon electrode (0.3 mm in diameter) was used as a receiver to prepare a nanofiber membrane biosensor. Using the electrode as the working electrode, the platinum wire electrode as the auxiliary electrode, and the saturated calomel electrode as the reference electrode, the response of the biosensor to the catechol solution was measured...

Embodiment 3

[0040] Preparation of glucose oxidase sensor

[0041] First prepare the acrylonitrile-acrylic acid copolymer into a 6% N,N-dimethylformamide solution, then use a phosphate buffer solution to configure an 8 mg / ml glucose oxidase solution, the polymer solution as the outer layer, and the enzyme solution As the inner layer, electrospinning is carried out at 15KV voltage, 15cm spinning distance, 40% humidity, and room temperature. The injection speed of the outer layer is set to 0.3mm / min, and the injection speed of the inner layer is set to 0.1mm / min. The spinning time is set to 40 s, and a conventional platinum electrode (0.2 mm in diameter) is used as a receiver to prepare a nanofiber membrane biosensor. Using the electrode as the working electrode, the platinum wire electrode as the auxiliary electrode, and the saturated calomel electrode as the reference electrode, the response of the biosensor to the glucose solution was measured in a phosphate buffer solution with a constan...

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Abstract

The invention discloses an electrostatic spinning method for preparing a nanofiber membrane biosensor. The method comprises the following steps: preparing a acrylonitrile-crylic acid copolymer into an N,N-dimethyl formamide solution as an outer-layer spinning solution, preparing enzyme into an enzyme solution as an inner-layer spinning solution, and exerting high voltage power to the solutions on an electrode to electrically spin into a fiber membrane so as to prepare the biosensor. By adopting the method, the defects that a conventional bioactive molecule such as immobilized enzyme is poor in accuracy, sensitivity, operation stability, service life, selectivity and the like in absorbing, cross-linking, covalent bonding, embedding and the like, are overcome; as the preparation method is very simple, the biosensor has great possibility of launching on the market. Biosensors of different functions can be prepared by using one same fixed material, are applicable to detection on various enzyme substrates, can be widely applied to fields of medicines, food, environment and the like, and have relatively high economic benefits.

Description

technical field [0001] The invention belongs to a method for preparing a biosensor by using a high molecular polymer as an outer layer and encapsulating enzyme active molecules in an inner layer by electrospinning a nanofiber membrane, and particularly relates to preparing a nanofiber membrane biosensor by using an electrospinning method. Background technique [0002] The basic principle of biosensors is based on the fact that biologically active substances have excellent molecular recognition functions and have high selectivity and sensitivity to the determination of substances; biologically active substances constitute the molecular recognition part, and the identified information is then converted into a detectable signal output. The core of the biosensor is mainly composed of two parts: molecular recognition and information conversion. The molecular recognition part refers to immobilized enzymes, microorganisms, antibodies, cells or tissues, etc., which is the core of th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
Inventor 薛怀国侯玉婷刘龙杰李丹阳虞惠艳
Owner 扬州智创企业运营管理服务有限公司
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