Breeding method for disease-resistant functional rice two-line sterile line
A functional, sterile line technology, applied in the direction of botany equipment and methods, applications, plant genetic improvement, etc., can solve the problem of low water-soluble protein content, achieve good nutritional and health effects, excellent agronomic and yield traits, Enhances gut health
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Embodiment 1
[0021] 1) Select high blast resistance variety "FR816" (A), low water-soluble protein rice variety "LGC21" (B), special rice "Zibao Xiangnuo 1" rich in natural vitamin A (C), high resistance "Jiangtangdao No. 1" (D), a functional rice variety with high starch content, was used for hybridization A / B and C / D to obtain hybrid F 1 Substituted by F 1-AB and F 1-CD express;
[0022] 2) Planting hybrid F 1 generation, crossed F at flowering stage 1-AB / F 1-CD , have to resubmit F 1 , with F 1-ABCD express;
[0023] 3) Plant F 1-ABCD F 2-ABCD , planting F 2-ABCD , with a population of 2,800 plants, identify rice blast resistance at the seedling stage, select and mark plants with high blast resistance, harvest seeds from individual plants at the mature stage, measure resistant starch content, water-soluble protein content, and vitamin A content, and obtain by screening F which is high in resistant starch, vitamin A and low in water soluble protein 3-ABCD seed;
[0024] 4)...
Embodiment 2
[0032] 1) Select high blast resistance variety "FR816" (A), low water-soluble protein rice variety "LGC21" (B), special rice "Zibao Xiangnuo 1" rich in natural vitamin A (C), high resistance "Jiangtangdao No. 1" (D), a functional rice variety with high starch content, was used for hybridization A / B and C / D to obtain hybrid F 1 Substituted by F 1-AB and F 1-CD express;
[0033] 2) Planting hybrid F 1 generation, crossed F at flowering stage 1-AB / F 1-CD , have to resubmit F 1 , with F 1-ABCD express;
[0034] 3) Plant F 1-ABCD F 2-ABCD , planting F 2-ABCD , with a population of 3,000 plants, identifying rice blast resistance at the seedling stage, selecting and marking plants with high blast resistance, harvesting seeds from individual plants at the mature stage, measuring the content of resistant starch, water-soluble protein, and vitamin A, and screening to obtain F which is high in resistant starch, vitamin A and low in water soluble protein 3-ABCD seed;
[003...
Embodiment 3
[0043] 1) Select high blast resistance variety "FR816" (A), low water-soluble protein rice variety "LGC21" (B), special rice "Zibao Xiangnuo 1" rich in natural vitamin A (C), high resistance "Jiangtangdao No. 1" (D), a functional rice variety with high starch content, was used for hybridization A / B and C / D to obtain hybrid F 1 Substituted by F 1-AB and F 1-CD express;
[0044] 2) Planting hybrid F 1 generation, crossed F at flowering stage 1-AB / F 1-CD , have to resubmit F 1 , with F 1-ABCD express;
[0045] 3) Plant F 1-ABCD F 2-ABCD , planting F 2-ABCD , with a population of 3,000 plants, identifying rice blast resistance at the seedling stage, selecting and marking plants with high blast resistance, harvesting seeds from individual plants at the mature stage, measuring the content of resistant starch, water-soluble protein, and vitamin A, and screening to obtain F which is high in resistant starch, vitamin A and low in water soluble protein 3-ABCD seed;
[004...
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