Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Charcoal-base immobilized lipase coated by nanogel modified by oleic acid molecule and preparation method of charcoal-base immobilized lipase

A technology for immobilizing lipase and nanogel, which is applied in directions such as being immobilized on or in an inorganic carrier, immobilized on/in an organic carrier, etc., and achieves the effects of mild conditions, simple preparation method and low cost

Active Publication Date: 2014-06-25
INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI +1
View PDF6 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Biochar is a low-cost, multifunctional and environmentally friendly material that has been widely used in the fields of greenhouse gas emission reduction, degraded soil improvement, and environmental remediation of heavy metals and organic pollutants. Currently, relevant biochar carriers are used to immobilize fat Enzymes have not been reported yet

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Charcoal-base immobilized lipase coated by nanogel modified by oleic acid molecule and preparation method of charcoal-base immobilized lipase
  • Charcoal-base immobilized lipase coated by nanogel modified by oleic acid molecule and preparation method of charcoal-base immobilized lipase
  • Charcoal-base immobilized lipase coated by nanogel modified by oleic acid molecule and preparation method of charcoal-base immobilized lipase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] The implementation of the present invention will be described in detail below in conjunction with specific examples, so as to fully understand and implement the process of how to apply technical means to solve technical problems and achieve technical effects in the present invention.

[0025] Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the reagents and products used are also commercially available. The sources, trade names and, where necessary, listing of constituents of reagents used are indicated the first time they appear.

[0026] Embodiments of the invention are:

[0027] refer to figure 1 , figure 2 , image 3 and Figure 4 , a preparation method of biochar-based immobilized lipase coated with oleic acid molecule-modified nanogel, the specific operation steps are as follows: 1) Preparation of biochar: After pulverizing thatch biomass, limit oxygen in a carbonization fu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of lipase immobilization and provides a preparation method of charcoal-base immobilized lipase coated by nanogel modified by an oleic acid molecule. The preparation method comprises the steps of carbonizing thatch biomass into a charcoal matrix, coating the surface of the charcoal matrix by virtue of the nanogel modified by the oleic acid molecule, adequately contacting the coated charcoal matrix with a lipase solution, and carrying out interface excitation, crosslinking fixation and freeze drying on the lipase, so as to obtain the immobilized lipase. The invention simultaneously provides the immobilized lipase prepared by utilizing the preparation method. The catalytic activity of the lipase prepared by utilizing the preparation method is substantially improved.

Description

technical field [0001] The invention relates to the technical field of lipase immobilization, in particular to a nanogel-coated biochar-based immobilized lipase modified by oleic acid molecules and a preparation method thereof. Background technique [0002] Bioenzymatic synthesis of biodiesel is recognized as an energy-saving and environment-friendly energy technology. The biological enzymatic method uses lipase as a catalyst to catalyze animal and vegetable oils (fatty acid triglycerides) and short-chain alcohols to synthesize fatty acid monoalkyl esters (biodiesel) in a non-aqueous solution system. This catalytic synthesis method has raw material pretreatment Simple, less alcohol consumption, low energy consumption, convenient product separation and recovery, and environmental friendliness. However, the catalytic activity and insufficient service life of free lipase limit its application in the catalytic production of biodiesel. Many strategies such as lipase molecular m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N11/14C12N11/04
Inventor 胡朝华万顺刚唐晓达张又弛
Owner INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com