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Charcoal-base immobilized lipase coated by nanogel modified by oleic acid molecule and preparation method of charcoal-base immobilized lipase

A technology for immobilizing lipase and nanogel, which is applied in directions such as being immobilized on or in an inorganic carrier, immobilized on/in an organic carrier, etc., and achieves the effects of mild conditions, simple preparation method and low cost

Active Publication Date: 2014-06-25
INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI +1
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Problems solved by technology

[0004] Biochar is a low-cost, multifunctional and environmentally friendly material that has been widely used in the fields of greenhouse gas emission reduction, degraded soil improvement, and environmental remediation of heavy metals and organic pollutants. Currently, relevant biochar carriers are used to immobilize fat Enzymes have not been reported yet

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  • Charcoal-base immobilized lipase coated by nanogel modified by oleic acid molecule and preparation method of charcoal-base immobilized lipase
  • Charcoal-base immobilized lipase coated by nanogel modified by oleic acid molecule and preparation method of charcoal-base immobilized lipase
  • Charcoal-base immobilized lipase coated by nanogel modified by oleic acid molecule and preparation method of charcoal-base immobilized lipase

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[0024] The implementation of the present invention will be described in detail below in conjunction with specific examples, so as to fully understand and implement the process of how to apply technical means to solve technical problems and achieve technical effects in the present invention.

[0025] Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the reagents and products used are also commercially available. The sources, trade names and, where necessary, listing of constituents of reagents used are indicated the first time they appear.

[0026] Embodiments of the invention are:

[0027] refer to figure 1 , figure 2 , image 3 and Figure 4 , a preparation method of biochar-based immobilized lipase coated with oleic acid molecule-modified nanogel, the specific operation steps are as follows: 1) Preparation of biochar: After pulverizing thatch biomass, limit oxygen in a carbonization fu...

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Abstract

The invention relates to the technical field of lipase immobilization and provides a preparation method of charcoal-base immobilized lipase coated by nanogel modified by an oleic acid molecule. The preparation method comprises the steps of carbonizing thatch biomass into a charcoal matrix, coating the surface of the charcoal matrix by virtue of the nanogel modified by the oleic acid molecule, adequately contacting the coated charcoal matrix with a lipase solution, and carrying out interface excitation, crosslinking fixation and freeze drying on the lipase, so as to obtain the immobilized lipase. The invention simultaneously provides the immobilized lipase prepared by utilizing the preparation method. The catalytic activity of the lipase prepared by utilizing the preparation method is substantially improved.

Description

technical field [0001] The invention relates to the technical field of lipase immobilization, in particular to a nanogel-coated biochar-based immobilized lipase modified by oleic acid molecules and a preparation method thereof. Background technique [0002] Bioenzymatic synthesis of biodiesel is recognized as an energy-saving and environment-friendly energy technology. The biological enzymatic method uses lipase as a catalyst to catalyze animal and vegetable oils (fatty acid triglycerides) and short-chain alcohols to synthesize fatty acid monoalkyl esters (biodiesel) in a non-aqueous solution system. This catalytic synthesis method has raw material pretreatment Simple, less alcohol consumption, low energy consumption, convenient product separation and recovery, and environmental friendliness. However, the catalytic activity and insufficient service life of free lipase limit its application in the catalytic production of biodiesel. Many strategies such as lipase molecular m...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/04
Inventor 胡朝华万顺刚唐晓达张又弛
Owner INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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