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Application of hirsutella sinensis refined fermentation liquor as bacteriostatic agent of bat moth infection paecilomyces farinosus

A Chinese technology of Trichosporum and bat moth larvae, which is applied to antifungal agents, medical preparations containing active ingredients, plant/algae/fungus/moss components, etc., and can solve the specific use of fermented liquid that does not clearly specify the mechanism of action, etc. question

Active Publication Date: 2014-06-25
YICHANG SHANCHENGSHUIDU CORDYCEPS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the antibacterial activity of Cordyceps militaris, Cordyceps sinensis, Trichoderma viride and Bamboo Yellow has been done preliminary research on the antibacterial activity, they only simply measured the inhibitory effect of the fermentation liquid on certain bacteria and fungi, and did not clearly specify the mechanism of action As well as the specific use of the fermented liquid, there is still a long way from the real use in industrial use

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Prepare the broth:

[0045] Mortierella chinensis was inoculated in the fermentation medium, the medium components were glucose 500g, peptone 150g, yeast powder 300g, anhydrous magnesium sulfate 10g, potassium dihydrogen phosphate 20g, water 25L, cultured at 15℃100rpm for 25d, obtained fermentation broth. After the fermentation broth was centrifuged, the supernatant was taken, rotatively evaporated at 40°C, and concentrated to 1 / 10 of the original volume to obtain a 10-fold refined solution.

[0046] Insect experiment:

[0047] Select 60 bat moth larvae infected with Mortierella sinensis, and spray 30 of them with Mortierella sinensis 15-fold fermented liquid to the surface of the larvae. The amount used is 50 mL per 100 worms. The other 30 strips were treated with sterile water as controls. It was treated every 15 days. After 4 months of treatment, 30 larvae in the treatment group were still healthy and alive. Microscopic examination of samples showed that there were ...

Embodiment 2

[0050] Prepare the broth:

[0051] Mortierella chinensis was inoculated in the fermentation medium, the medium components were glucose 800g, peptone 300g, yeast powder 150g, anhydrous magnesium sulfate 15g, potassium dihydrogen phosphate 30g, water 25L, cultured at 20°C 100rpm for 14d, obtained fermentation broth. After the fermentation broth was centrifuged, the supernatant was taken, rotatively evaporated at 50°C, and concentrated to 1 / 15 of the original volume to obtain a 15-fold refined solution, weighed and then added Tween 80 20g, that is, the mass fraction of Tween 80 was 1.2%.

[0052] Insect experiment:

[0053] Select 60 bat moth larvae infected with Mortierella sinensis, and spray 30 of them with Mortierella sinensis 30-fold fermented and refined solution to the surface of the larvae, with an amount of 100 mL per 100 worms. The other 30 strips were treated with sterile water as controls. It was treated every 15 days. After 4 months of treatment, 30 larvae in the ...

Embodiment 3

[0056] Prepare the broth:

[0057] Mortierella chinensis was inoculated in the fermentation medium, the medium components were glucose 750g, peptone 250g, yeast powder 250g, anhydrous magnesium sulfate 12.5g, potassium dihydrogen phosphate 25g, water 25L, cultured at 18°C ​​100rpm for 20d, Obtain a fermented broth. After the fermentation broth was centrifuged, the supernatant was taken, evaporated at 45°C, and concentrated to 1 / 10 of the original volume to obtain a 10-fold refined solution, which was weighed and then 20 g of PEG was added to it, that is, the mass fraction of PEG was 0.8%.

[0058] Insect experiment:

[0059] Select 60 bat moth larvae infected with Mortierella sinensis, and spray 30 of them with Mortierella sinensis 20-fold fermented and refined solution to the surface of the larvae, with an amount of 75 mL per 100 worms. The other 30 strips were treated with sterile water as controls. It was treated every 15 days. After 4 months of treatment, 30 larvae in t...

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Abstract

The invention relates to the field of prevention and control of animal diseases, and in particular relates to application of hirsutella sinensis refined fermentation liquor as a bacteriostatic agent of bat moth infection paecilomyces farinosus, a paecilomyces farinosus bacteriostatic agent and a preparation method of the paecilomyces farinosus bacteriostatic agent. The bacteriostatic agent provided by the invention can be used for well inhibiting paecilomyces farinosus cultured in vitro and / or in vivo without causing any bad influence on bat moths. Although the bacteriostatic agent is applied continuously to infected moths, hirsutella sinensis in the moths can still live till the moths become stiff. Moreover, stiff moths can form a sporocarp blastema.

Description

[0001] This application requires a Chinese patent submitted to the China Patent Office on December 19, 2012, with the application number 201210554669.6, and the title of the invention is "Application of Chinese Mortierella Fermented Liquid Refined Liquid as a Bacteriostatic Agent for Bat Moth Larvae Infected with Paecilomyces" The priority of the application, the entire content of which is incorporated in this application by reference. technical field [0002] The invention relates to the field of animal disease prevention and control, in particular to the application of the refined liquid of Mortierella chinensis fermentation liquid as a bacteriostatic agent for Paecilomyces infecting bat moth larvae. Background technique [0003] Cordyceps sinensis is a famous and precious Chinese herbal medicine in China, and it is listed as the three major Chinese tonics together with ginseng and velvet antler. As early as 1757, in "Materia Medica Congxin", there is a record that "Cordyc...

Claims

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Application Information

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IPC IPC(8): A61K36/068A61P31/10
Inventor 梁蕾李光荣梁关海朱志钢李文佳谢俊杰
Owner YICHANG SHANCHENGSHUIDU CORDYCEPS
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