Method for intensively extracting polysaccharides from rice culture medium for cordyceps militaris

Abstract

The invention discloses a method for intensively extracting polysaccharides from a rice culture medium for cordyceps militaris. The method comprises the following steps: carrying out superfine crushing, ultrahigh pressure treatment and ultrasonic intensified flash extraction on the cordyceps militaris culture medium, adding amylase to an extract solution to realize enzymosis, concentrating enzymatic hydrolysate, adding polyethylene glycol, sufficiently stirring and dissolving, standing over night, centrifuging, washing precipitate with edible alcohol, adding hot water to dissolve, removing insoluble substances, and drying to obtain the polysaccharides. According to the method disclosed by the invention, configuration guiding of a process for promoting effective dissolution of polysaccharides is highlighted according to the resource advantage of the rice culture medium for cordyceps militaris, the clean production ideal is embodied by technical model selection, by adopting novel physical and biological integrated means, polysaccharide recovery technical output characterized by high speed, high efficiency, time saving, low consumption and environmental friendliness is realized, and the edible safety of the extracted polysaccharides is ensured from the production link.

Description

technical field [0001] The invention belongs to the technical field of preparation of polysaccharides, and in particular relates to a method for intensively extracting polysaccharides from Cordyceps militaris rice medium. Background technique [0002] Cordyceps militaris, also known as Cordyceps militaris, is a traditional precious nourishing Chinese herbal medicine, which is listed as the three major tonics together with natural ginseng and antler. It has mild medicinal properties and can be eaten all year round. It is suitable for the old, young, sick, weak, and weak. It has wider medicinal value than other types of tonics. People have used it as a tonic and medicinal product since ancient times. Natural wild Cordyceps mainly grows in high-altitude and high-cold regions, and the composition of the growing environment is very harsh and complex. In addition, people's demand for Cordyceps continues to increase and unreasonable excavation, resulting in the gradual reduction o...

AI Technical Summary

Problems solved by technology

(2) Most of the polysaccharide extraction and purification processes involved in the medium are simple and lack innovation, and the extraction rate is falsely high

In combination with the existing published literature, it can be known that the disadvantages of the polysaccharide extraction of the Cordyceps culture medium involved are low technical content, high energy consumption, and more organic and toxic chemical reagents are introduced in some links. The most prominent disadvantages are Impurity factors such as starch were not considered during the extraction process, resulting in falsely high values ​​of the measured polysaccharides

(3) Judging from the existing literature reports on the polysaccharides of Cordyceps culture medium, the purification techniques used can be summarized into water extraction, water extraction and alcohol precipitation, alcohol extraction, column chromatography (resin adsorption, activated carbon adsorption, particle exchange Adsorption, etc.), membrane separation and purification, etc., its shortcomings are as follows: some technical focus is not prominent, and other active ingredients (such as cordycepin) are taken into account while extracting polysaccharides; some extraction methods are simple and single, and the investment and energy consumption are relatively large. The implementation of the green and clean production process requires the use of a large amount of organic and other chemical reagents, and brings waste gas and waste water, causing environmental pollution and is not conducive to the retention of polysaccharide activity; its common disadvantage is that there is no mycelium wrapping for the Cordyceps culture medium. The formed macro-agglomerates and polysaccharide components are located in the cell wall to optimize the applicability of the wall-breaking dissolution technology, which makes the extraction insufficient and the loss of active ingredients is large, resulting in a lot of waste and loss.