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Latex immune enhancement turbidimetric kit for detecting peptide C quantitatively

An immunoturbidimetric and latex-enhanced technology, which is applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of unstable test results, high equipment requirements, and low degree of automation, so as to improve accuracy and credibility , reduce production costs, and achieve a high degree of automation

Active Publication Date: 2014-06-11
CHONGQING ZHONGYUAN BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005]Currently, clinical methods for measuring C-peptide mainly include radioimmunoassay (RIA), enzyme-linked immunosorbent assay (Enzyme-Linked, Immunosorbent Assay, ELISA) and chemiluminescence methods, among which RIA method has high sensitivity, but it has high requirements for equipment, unstable test results and radioactive pollution; ELISA has poor quantitative accuracy, long operation time and low degree of automation, which cannot meet the needs of large numbers of patients in clinical practice. The need for rapid detection; the chemiluminescence method has the disadvantages of expensive instrument maintenance, susceptible to interference, and poor repeatability

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  • Latex immune enhancement turbidimetric kit for detecting peptide C quantitatively
  • Latex immune enhancement turbidimetric kit for detecting peptide C quantitatively
  • Latex immune enhancement turbidimetric kit for detecting peptide C quantitatively

Examples

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Embodiment 1

[0038] A latex-enhanced immunoturbidimetric kit for quantitative detection of C-peptide, including C-peptide R1 reagent, C-peptide R2 reagent and C-peptide calibrator.

[0039] Taking the preparation of 1L C-peptide R1 reagent as an example, it is prepared from Tris-HCl buffer solution with a concentration of 50mM, 3g bovine serum albumin BSA, 1g sodium azide and 50g PEG6000, and the final content of Tris-HCl buffer solution in C-peptide R1 reagent The concentration is 50mM, the concentration of bovine serum albumin BSA is 3g / L, the concentration of sodium azide is 1g / L, and the concentration of PEG6000 is 50g / L;

[0040] Taking the preparation of 1L C-peptide R2 reagent as an example, the concentration is 50mM Tris-HCl buffer solution, 5g of bovine serum albumin BSA, 1g of sodium azide and sensitized polystyrene latex particles coated with anti-human C-peptide antibody Prepared, the concentration of Tris-HCl buffer in the final C-peptide R2 reagent is 50mM, the concentration ...

Embodiment 2

[0056] A latex-enhanced immunoturbidimetric kit for quantitative detection of C-peptide, including C-peptide R1 reagent, C-peptide R2 reagent and C-peptide calibrator.

[0057] Taking the preparation of 1L of C-peptide R1 reagent as an example, it is prepared from 200mM glycine buffer solution, 5g bovine serum albumin BSA, 2g sodium azide and 40g PEG8000, and the final concentration of glycine buffer solution in C-peptide R1 reagent is 200mM. The concentration of serum albumin BSA is 5g / L, the concentration of sodium azide is 2g / L, and the concentration of PEG8000 is 40g / L;

[0058] Taking the preparation of 1L C-peptide R2 reagent as an example, it is prepared from borax buffer solution with a concentration of 50mM, 5g of bovine serum albumin BSA, 0.3g of sodium azide and sensitized polystyrene latex particles coated with anti-human C-peptide antibody Obtain, the concentration of borax buffer solution in the final C peptide R2 reagent is 50mM, the concentration of bovine seru...

Embodiment 3

[0062] A latex-enhanced immunoturbidimetric kit for quantitative detection of C-peptide, including C-peptide R1 reagent, C-peptide R2 reagent and C-peptide calibrator.

[0063] Taking the preparation of 1L C-peptide R1 reagent as an example, it is prepared from PBS buffer solution with a concentration of 200mM, 5g bovine serum albumin BSA, 3g sodium azide and 50g PEG8000, and the final concentration of PBS buffer solution in the C-peptide R1 reagent is 200mM. The concentration of bovine serum albumin BSA is 5g / L, the concentration of sodium azide is 3g / L, and the concentration of PEG8000 is 50g / L;

[0064] Taking the preparation of 1L C-peptide R2 reagent as an example, it is prepared from 50mM borax buffer solution, 5g bovine serum albumin BSA, 1g sodium azide and sensitized polystyrene latex particles coated with anti-human C-peptide antibody , the concentration of borax buffer in the final C-peptide R2 reagent is 50mM, the concentration of bovine serum albumin BSA is 5g / L, ...

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Abstract

The invention discloses a latex immune enhancement turbidimetric kit for detecting peptide C quantitatively. The latex immune enhancement turbidimetric kit for detecting peptide C quantitatively consists of a peptide C R1 reagent, a peptide C R2 reagent and a peptide C calibration product, wherein the peptide C R1 reagent comprises a buffer liquid, a protective agent I, a reaction enhancer and preservatives; the peptide C R2 reagent comprises a buffer liquid, a protective agent I, preservatives and sensitization polystyrene latex particles coated with anti-human peptide C antibodies; the peptide C calibration product comprises a buffer liquid, a protective agent II, preservatives and peptide C recombinant protein. The kit prepared by the invention is suitable for semi-automatic and full-automatic biochemical analyzers and scattering turbidimetric analyzers, has the advantages of simple and rapid operation, high testing accuracy and high automation degree, is suitable for being used clinically and widely, and is especially suitable for rapid and quantitative determination of emergency treatment.

Description

technical field [0001] The invention belongs to the field of in vitro diagnostic reagents, and in particular relates to a kit for quantitatively measuring and detecting C-peptide content in specimens by using latex-enhanced immune turbidimetry. Background technique [0002] C-peptide, also known as connecting peptide, is composed of 31 amino acids and has a molecular weight of 3020 Daltons. It is a short peptide connecting insulin A chain and B chain in proinsulin, and its function is to promote insulin molecules in β cells. Synthesized in the endoplasmic reticulum and enables efficient folding and assembly of insulin. Clinically, the determination of C-peptide is mainly used to correctly evaluate the functional changes and development rules of islet β cells, as well as to accurately determine the type of diabetes and monitor the condition. [0003] Human pancreatic β-cells synthesize proinsulin, which is subsequently cleaved equimolarly into insulin and C-peptide. Therefo...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/545G01N33/6803G01N2333/62
Inventor 李民友吉权
Owner CHONGQING ZHONGYUAN BIOLOGICAL TECH
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