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A Triethylamine Degrading Bacteria and Its Application

A technology of ethylamine degrading bacteria and triethylamine, which is applied in bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., can solve problems that are not involved in practical industrial applications, and achieve the effect of improving COD removal rate

Active Publication Date: 2016-05-25
SHANDONG NEWTIME PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese invention patent CN101935625A discloses a triethylamine-degrading bacterium and the bacterial agent produced by it. The bacterium is only used in laboratory shake flasks and does not involve actual industrial application

Method used

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  • A Triethylamine Degrading Bacteria and Its Application
  • A Triethylamine Degrading Bacteria and Its Application
  • A Triethylamine Degrading Bacteria and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: the cultivation method of triethylamine degrading bacteria T-5

[0032] The bacterium T-5 of the present invention was inoculated in 10 mL of proliferation medium, and placed in a constant temperature shaking shaker at 25°C and pH 7.5 for 3 days to obtain a T-5 bacterial suspension. for 10 9 individual / mL. Spread the T-5 bacterial suspension on a solid medium plate, and store it as a bacterial strain at 4°C after the colony grows.

[0033] Wherein, the composition of the proliferation medium is: KH 2 PO 4 , 0.09; K 2 HPO 4 , 0.22; NaH 2 PO 4 , 0.26; MgSO 4 ·7H 2 O, 0.23; CaCl 2 , 0.28; FeCl 3 , 0.003; triethylamine, 1.5 (unit: g / L).

[0034] Described solid medium composition is: KH 2 PO 4 , 0.09; K 2 HPO 4 , 0.22; NaH 2 PO 4 , 0.26; MgSO 4 ·7H 2 O, 0.23; CaCl 2 , 0.28; FeCl 3 , 0.003; triethylamine, 1.0; agar, 15-20. (Unit: g / L).

Embodiment 2

[0035] Embodiment 2: the expanding culture method of triethylamine degrading bacteria T-5

[0036] Take 2 mL of the T-5 bacterial suspension in Example 1 and inoculate it into 200 mL of proliferation medium for expanded culture, and place it in a constant temperature shaking shaker for 3 days at 35° C. and a pH of 5.5 to obtain T-5 bacterial suspension. Suspension, the number of bacteria is 10 9 individual / mL. Take 20 mL of the above-mentioned T-5 bacterial suspension and inoculate it into 2000 mL of proliferation medium for expansion culture, and place it in a constant temperature shaking shaker for 3 days under the conditions of 30 °C and pH 7.0 to obtain the T-5 bacterial suspension. Body count reaches 10 9 individual / mL.

Embodiment 3

[0037] Embodiment 3: T-5 is to the shaking flask experiment of 1500mg / L waste water

[0038] Get 2 mL of the T-5 bacterial suspension cultivated in Example 1 and inoculate it into 200 mL of wastewater with a triethylamine concentration of 1500 mg / L, cultivate at a constant temperature of 30° C., pH is 7.0, and react for 72 hours. Take 10mL of the bacterial suspension and centrifuge at 3000rpm for 5min, then take the supernatant for measurement, no triethylamine was detected.

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a strain of triethylamine degrading bacterium and application thereof. The triethylamine degrading bacterium T-5 belongs to bacillus, and is collected in the China Center for Type Culture Collection on 21 November, 2013 with the collection number CCTCC NO:M2013588. The bacterial strain can grow and breed by taking triethylamine as a carbon source as well as a nitrogen source, and can grow and breed in a shake flask containing 4,000mg / L triethylamine, and the degrading rate of 1,500mg / L triethylamine within 72 hours is 100 percent. During treatment of industrial waste water containing high-concentration triethylamine, when the concentration of triethylamine is lower than 2,000mg / L, the removing rate of triethylamine is up to 100 percent, and the COD (Chemical Oxygen Demand) removing rate is increased by 30-40 percent. The bacterial strain can be applied to biological enhancement treatment of pharmaceutical waste water and pesticide processing waste water, and is wide in application prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a triethylamine-degrading bacterium and its application. Background technique [0002] Triethylamine is an important raw material for organic solvents and chemical synthesis. It is used as a catalyst for polycarbonate phosgene, a polymerization inhibitor for tetrafluoroethylene and a rubber vulcanization accelerator in the manufacture of synthetic resins; it can also be used as a food preservative and emulsifier. ; At the same time, it is also a raw material for the manufacture of fuels and pesticides. Triethylamine is an aliphatic amine with the molecular formula (C2H5) 3 N, with strong ammonia odor, irritating, flammable and volatile. [0003] Triethylamine is widely found in organic synthesis, pesticide, pharmaceutical, fuel and other production wastewater. At present, physical and chemical methods are mostly used for the treatment of triethylamine-containing wastewa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/085C02F101/38
Inventor 赵志全许尚营苏建文王彩冬王俊超
Owner SHANDONG NEWTIME PHARMA
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