Method for extracting anti-diabetic effective part of olive leaf by adopting macro-porous resin adsorption

A technology of olive leaf and macroporous resin is applied in the field of using macroporous resin to absorb and extract effective anti-diabetic parts of olive leaf, and can solve problems such as large side effects, cross allergy, hyperlactatemia and the like

Inactive Publication Date: 2014-06-04
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

According to its chemical characteristics, it is mainly divided into two categories, that is, sulfonylureas, such as glyburide, mepyrid, tosulfuron, ketangli, etc., but long-term use of these drugs damages the liver, kidneys and blood cells, and sulfonamides Drug cross allergy, secondary failure occurs in the later stage; biguanides, such as Jiangtangling, Jiangtang Tablets, etc., but these drugs have low curative effect and large side effects, and long-term use can cause hyperlactic acidemia
In recent years, a lot of research has been done on Chinese medicines for hypoglycemia, and compound Chinese medicine preparations such as "Tangmaikang" have appeared, but the ingredients are clear, and there are no Chinese medicine varieties that can enter the international market.

Method used

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  • Method for extracting anti-diabetic effective part of olive leaf by adopting macro-porous resin adsorption
  • Method for extracting anti-diabetic effective part of olive leaf by adopting macro-porous resin adsorption
  • Method for extracting anti-diabetic effective part of olive leaf by adopting macro-porous resin adsorption

Examples

Experimental program
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Effect test

Embodiment 1

[0054] Take the dried leaves of olive oil, add 6 times the amount of 40% ethanol, heat and reflux for extraction 3 times, 2 h each time, filter, combine the extracts, concentrate to 0.25 g / mL equivalent to the original medicinal material, and obtain the sample solution; The sample solution is absorbed by a chromatographic column filled with macroporous resin, and the resin is eluted with water for 3 BV, the elution flow rate is 4 BV / h, and the water eluent is discarded; then gradient elution is carried out with different concentrations of ethanol aqueous solution 5 BV, the flow rate was 4 BV / h, and the ethanol eluents were collected separately; the eluents were concentrated under reduced pressure and dried in vacuum to obtain different sample active sites, of which the D site was the anti-diabetic active site.

Embodiment 2

[0056] Take the dried leaves of olive oil, add 8 times the amount of 80% ethanol, heat and reflux for extraction twice, each time for 2 hours, filter, combine the extracts, concentrate to 0.3 g / mL equivalent to the original medicinal material, and obtain the sample solution; The sample solution is absorbed by a chromatographic column filled with macroporous resin, and the resin is eluted with water at 4 BV, the elution flow rate is 4 BV / h, and the water eluent is discarded; then gradient elution is carried out with different concentrations of ethanol aqueous solution 6 BV, the flow rate was 4 BV / h, and the ethanol eluate was collected separately; the eluate was concentrated under reduced pressure and dried in vacuum to obtain different active sites of the sample, among which the D site was the anti-diabetic active site.

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Abstract

The invention discloses a method for extracting an anti-diabetic effective part of olive leaf by adopting macro-porous resin adsorption. The method comprises the following steps: carrying out heating reflux extraction of olive leaf by an aqueous ethanol solution, mixing above obtained extract liquids, and concentrating the above obtained liquid mixture to form a concentrate to be used as an introduced sample liquid; and allowing the introduced sample liquid to go through a macro-porous resin column to realize adsorption, washing the resin column after the adsorption in order to remove water-soluble impurities, carrying out gradient elution by different concentrations of the aqueous ethanol solution to realize desorption, respectively connecting ethanol desorption liquids obtained after the elution using different concentrations of ethanol to obtain a part A, a part B, a part C, a part D and a part E of olive leaf, wherein the part D is the anti-diabetic active part of olive leaf The natural anti-diabetic effective component screened by utilizing the macro-porous resin has a high content and a substantial sugar reducing effect.

Description

technical field [0001] The invention relates to a method for absorbing and extracting effective anti-diabetic parts of olive leaves by using macroporous resin. Background technique [0002] diabetes( diabetes ) is caused by genetic factors, immune dysfunction, microbial infection and its toxins, free radical toxins, mental factors and other pathogenic factors acting on the body to cause hypofunction of islets, insulin resistance, etc. caused by sugar, protein, fat, water Clinically, hyperglycemia is the main feature. Typical cases may show polyuria, polydipsia, polyphagia, weight loss, etc., that is, "three more and one less" symptoms. Once diabetes (blood sugar) Poor control will lead to complications, leading to failure of the kidneys, eyes, feet and other parts of the disease, and can not be cured. [0003] According to the report of China Diabetes Information Network, there are currently more than 100 million diabetics in the world, of which nearly 20 million are in my...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/63A61P3/10A61K127/00
Inventor 邸多隆黄新异
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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