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Liquid fermentation method for improving yield of phellinus fermentation mycelia

A technology of fermentation mycelium and liquid fermentation, which is applied in the field of liquid fermentation for improving the yield of Phellinus fermented mycelium, can solve the problems of long fermentation period, limited improvement of fermentation level, low production efficiency, etc. The effect of high mycelium yield

Inactive Publication Date: 2014-05-28
MICROBIOLOGY INST OF SHAANXI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, some scientific research institutes and biological companies are producing Phellinus mycelium by fermentation, but there is no analysis and research from the perspective of Phellinus biological metabolism. The improvement of the fermentation level of Phellinus mycelia produced by fermentation is limited, and the fermentation cycle Long, high cost, low overall production efficiency, far from meeting the needs of further industrialized fermentation production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Step 1: inoculate Phellinus bacterium into liquid culture medium, carry out activation culture, then receive in the seed fermentation medium by volume percentage 13% inoculum amount, carry out seed culture, seed culture condition is: temperature 28 ℃, Cultivate on a shaking table for 108 hours to obtain a seed solution;

[0024] Step 2: Inoculate the seed liquid obtained in Step 1 into the liquid fermentation medium at a volume ratio of 10-15% inoculum. Under the condition of a temperature of 27-30°C, the liquid fermentation culture is carried out for 24 hours, and then the abscisic acid solution is added to shed The concentration of acid is 0.5ppm / L, the concentration of abscisic acid is the whole solution concentration in step 2, then continue to cultivate for 72h, obtain the mixed solution of Phellinus mycelium and fermented liquid;

[0025] Step 3: Filter the mixture of mycelium and fermentation broth obtained in Step 2 through a plate-and-frame filter press, dry th...

Embodiment 2

[0027] Step 1: inoculate Phellinus bacterium into liquid culture medium, carry out activation culture, then receive in the seed fermentation medium by volume percentage 13% inoculum amount, carry out seed culture, seed culture condition is: temperature 28 ℃, Cultivate on a shaking table for 108 hours to obtain a seed solution;

[0028] Step 2: Inoculate the seed liquid obtained in Step 1 into the liquid fermentation medium at a volume ratio of 10-15% inoculum. Under the condition of a temperature of 27-30°C, ferment the liquid for 30 hours, then add the abscisic acid solution to shed The concentration of the acid is 1.0ppm / L, the concentration of the abscisic acid is the concentration of the entire solution in step 2, and then the culture is continued for 62h to obtain the mixed solution of Phellinus mycelia and fermentation broth;

[0029] Step 3: Same as Example 1.

Embodiment 3

[0031] Step 1: inoculate Phellinus bacterium into liquid culture medium, carry out activation culture, then receive in the seed fermentation medium by volume percentage 13% inoculum amount, carry out seed culture, seed culture condition is: temperature 28 ℃, Cultivate on a shaking table for 108 hours to obtain a seed liquid;

[0032] Step 2: Inoculate the seed solution obtained in Step 1 into the liquid fermentation medium at a volume ratio of 10-15% inoculum, and culture the liquid for 36 hours at a temperature of 27-30°C, and then add abscisic acid solution to shed The concentration of acid is 1.5ppm / L, the concentration of abscisic acid is the whole solution concentration in step 2, then continue to cultivate for 72h, obtain the mixed solution of Phellinus mycelium and fermented liquid;

[0033] Step 3: Same as Example 1.

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Abstract

The invention belongs to the technical field of bio-fermentation engineering, and particularly relates to a liquid fermentation method for improving the yield of phellinus fermentation mycelia. The fermentation cycle of the liquid fermentation method is short, and the yield of the fermentation mycelia is high. The technical scheme adopted by the invention is that the liquid fermentation method comprises the following steps: inoculating phellinus strains into a liquid medium for activation cultivation, and then inoculating into a seed fermentation medium based on an inoculum size of 13% of the volume percentage for seed cultivation to obtain a seed solution; inoculating the seed solution into a liquid fermentation medium based on an inoculum size of 10-15% of the volume percentage of the seed solution for liquid fermentation cultivation at 27-30 DEG C for 24-36 h, then adding an abscisic acid solution for cultivation for 56-72 h to obtain the phellinus mycelia and a fermentation solution; filter-pressing the mycelia and the fermentation solution obtained through a plate-and-frame filter press, drying filter cakes at 70-80 DEG C, spray drying the filtrate after vacuum concentration into dry powders through a spray dryer, and mixing the filter cakes with the dry powders to obtain the phellinus fermentation mycelia.

Description

1. Technical field: [0001] The invention belongs to the technical field of biological fermentation engineering, and in particular relates to a liquid fermentation method for increasing the yield of Phellinus fermented mycelium. 2. Background technology: [0002] Phellinus is a perennial medicinal fungus, and it is one of the most promising rare and endangered traditional Chinese medicines in China. It is mainly distributed in the "Ziwuling" area at the junction of Qinling, Shaanxi and Gansu. It grows on dead standing trees and trunks of broad-leaved trees such as poplar, mulberry, willow, birch, zelkova, and peach. "Dictionary of Chinese Medicine" records that it can cure gynecological diseases such as metrorrhagia, bloody stranguria, leukorrhea, and amenorrhea. Modern studies have found that Phellinus has obvious anti-hepatic fibrosis effects, and is currently internationally recognized as the highest effective rate in the field of biological cancer treatment of higher fu...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
Inventor 雷萍吴亚召张文隽安军民陈旭吕德平
Owner MICROBIOLOGY INST OF SHAANXI
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