High-density culture technology for oocystis borgei and collection method for oocystis borgei cells

Abstract

The invention discloses a high-density culture technology for oocystis borgei and a collection method for oocystis borgei cells, and relates to the field of cultivation of marine microalgae, and the traditional culture medium for oocystis borgei is slow in growth rate and low in output. According to the invention, NaNO3 and urea are used as nitrogen sources, proper amount of NaHCO3 is added and 920 plant growth substance, human urine, sea mud extract liquid, cow dung and sheep manure lixivium and soil extract are supplemented, and the nutrition is rather balanced and comprehensive; a domestic used alkaline mineral water bucket culture method replaces the traditional open cement pool culture method, the operation is simple, the possibility of pollution is low, control is easy, and the method is safe and reliable, production is available at all seasons, the cost is low, the growth is quick, the method is particularly suitable for the plant expanding phase, the yield is improved by 180%; the yellow mud method for the collection of oocystis borgei replaces the original centrifugation method, the cell recovery rate is high, the method is simple and feasible, costs little, causes no damage to cells, is suitable for large-scale production and high in economic benefit, the equipment is simple and materials are easily obtained.

Description

technical field [0001] The invention belongs to the field of marine microalgae cultivation, and in particular relates to a method for high-density cultivation of oocysts boggy and a method for collecting algae cells. Background technique [0002] Oocystis borgei is isolated from the high-level pond of prawns. It is used as a microalgae for microalgae ecological regulation, improvement of the breeding environment, and prevention of prawn diseases. It belongs to the Chlorophyta, Chlorophyceae, Chlorophyceae Chlorococcales, Oocystaceae Oocystaceae, Oocystis Oocystis. Live in groups consisting of 2, 4, 8 cells, the group is oval; there is a layer of obvious gelatin coating outside the group, the cells in the group are ellipse or slightly oval, with wide round ends; the pigment body is sheet-like. The cells are 10-19 μm long and 9-13 μm wide; generally produce 2, 4, 8 or 16 spore-like spores, which are spore-like. [0003] The high level pond is a high-density intensive culture...

AI Technical Summary

Problems solved by technology

[0007] Regarding the method of collecting Oocystella boggie cells, the most commonly used method in the past was centrifuge centrifugation, no pollution, more thorough centrifugation, but the disadvantages are too obvious: centrifugation equipment is expensive, even a simple large centrifuge needs 400,000-50 10,000 yuan, not only consumes a lot of electricity, but also has high operating costs, and it will break algae cells and reduce production

Some people also use alum and lime water precipitation method to collect algae cells, the recovery efficiency is higher, but alum and lime have pollution and damage to algae cells, and the cost is very high

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