Real-time fluorescent PCR detection system based on rotary microfluidic chip
A real-time fluorescence and detection system technology, applied in the fields of biology, analytical chemistry and medical detection, can solve the problems of increasing the difficulty of miniaturization, the large size of the fluorescence microspectroscopy detection system, and the low implementation efficiency, so as to avoid PCR reagents hanging up. The effect of improving the efficiency of experimental processing and facilitating the detection of samples
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[0030] The present invention will be further described in detail below with reference to the accompanying drawings.
[0031] A real-time fluorescent PCR detection system based on a rotary microfluidic chip, the system includes an annealing low temperature zone 1, an extended temperature zone 2, a thermally variable high temperature zone 3, a rotating module 4, a PCR reagent chamber 5, an injection microchannel 6, Instrument fixed base 7, fluorescence detection system 8; among them, the temperature cycle control module includes annealing low temperature zone 1, extended temperature zone 2, thermal variable high temperature zone 3; microchannel circulation PCR amplification module includes rotation module 4, PCR reagent containing chamber 5. Injection microchannel 6, instrument fixing base 7; the fluorescence spectrum detection module includes a fluorescence detection system 8.
[0032] The three temperature zones of the annealing low temperature zone 1, the extended temperature...
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