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Real-time fluorescent PCR detection system based on rotary microfluidic chip

A real-time fluorescence and detection system technology, applied in the fields of biology, analytical chemistry and medical detection, can solve the problems of increasing the difficulty of miniaturization, the large size of the fluorescence microspectroscopy detection system, and the low implementation efficiency, so as to avoid PCR reagents hanging up. The effect of improving the efficiency of experimental processing and facilitating the detection of samples

Inactive Publication Date: 2015-12-30
BEIJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the current market, common real-time fluorescent PCR detection instruments cannot meet the needs of the space field, and mainly have the following shortcomings:
[0006] 1. The temperature control module in the PCR amplification system in the instrument uses a metal block for heating and fan cooling. The entire system consumes a lot of power and is too bulky to be miniaturized. Handling of problems and requirements for rapid testing of samples;
[0007] 2. The volume of the photomultiplier tube or the charge-coupled element in the fluorescence detection system in the instrument is very large, and it is used separately, which requires a supporting optical path system, resulting in a large volume of the entire fluorescence micro-spectrum detection system, which increases the miniaturization
Cannot be applied in the rapidly developing space field;
[0008] 3. The transmission of excitation light and the collection of reflected light in the transmission system in the instrument require an optical path composed of various optical devices and optical fibers for optical transmission. Not only is the structure complex and difficult to miniaturize and integrate, but it also affects the sensitivity of each module in real-time fluorescence detection. and stability;
[0009] 4. The current instrument is not only bulky but also unable to monitor and feedback the temperature change in the microchannel, the flow rate of the microfluid and the fluorescence signal in real time;
[0010] 5. The medium temperature control system of small instruments lacks complete isolation, and the three temperature zones are easy to interfere with each other, which seriously affects the biological reaction results;

Method used

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  • Real-time fluorescent PCR detection system based on rotary microfluidic chip
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  • Real-time fluorescent PCR detection system based on rotary microfluidic chip

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Embodiment Construction

[0030] The present invention will be further described in detail below with reference to the accompanying drawings.

[0031] A real-time fluorescent PCR detection system based on a rotary microfluidic chip, the system includes an annealing low temperature zone 1, an extended temperature zone 2, a thermally variable high temperature zone 3, a rotating module 4, a PCR reagent chamber 5, an injection microchannel 6, Instrument fixed base 7, fluorescence detection system 8; among them, the temperature cycle control module includes annealing low temperature zone 1, extended temperature zone 2, thermal variable high temperature zone 3; microchannel circulation PCR amplification module includes rotation module 4, PCR reagent containing chamber 5. Injection microchannel 6, instrument fixing base 7; the fluorescence spectrum detection module includes a fluorescence detection system 8.

[0032] The three temperature zones of the annealing low temperature zone 1, the extended temperature...

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Abstract

The invention relates to a real-time fluorescence PCR (polymerase chain reaction) detection system based on a rotary type microfluidic chip. The system comprises an annealing low-temperature area, an extension appropriate temperature area, a thermal change high temperature area, a rotating module, a PCR reagent accommodating cavity, an injection micro-channel, an instrument fixing base and a fluorescence detection system, wherein a temperature cycle control module comprises the annealing low-temperature area, the extension appropriate temperature area and the thermal change high temperature area; a micro-channel cycle PCR amplification module comprises the rotating module, the PCR reagent accommodating cavity, the injection micro-channel and the instrument fixing base; and a fluorescence spectrum detection module comprises the fluorescence detection system. According to the system, three modules, namely, the micro-channel cycle PCR amplification module, the temperature cycle control module and the fluorescence spectrum detection module are integrated, so that the portable micro PCR fluorescence real-time detection system applicable to space operation requirements is realized, and purposes of function integration, structure microform, lightness, small size and full-automatic detection are achieved.

Description

technical field [0001] The invention relates to a real-time fluorescent PCR detection system based on a rotary microfluidic chip, belonging to the fields of biology, analytical chemistry and medical detection. Background technique [0002] At present, in space vehicles during long-term space flight, the growth of microbial flora and strain variation will affect the performance of instruments and equipment and working environment conditions, and cause harm to the normal operation of spacecraft and its instruments and equipment. In order to prevent the occurrence, spread and effective treatment of infectious diseases in space flight, a real-time automatic alarm system for space on-orbit biological hazards is urgently needed, and its core is a microbial nucleic acid fluorescence detection microsystem. Due to the space on-orbit, the equipment is required to have the technical characteristics of full automation, small volume and small weight. Therefore, simplifying the microflui...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/38C12M1/36C12M1/34
CPCB01L3/5027B01L7/5255G01N21/6486
Inventor 吴坚于伦王富吉陈涛郑杨
Owner BEIJING UNIV OF TECH
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