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Method for preparing highly unsaturated camellia seed clean oil by using biological enzyme method

A biological enzyme method and Camellia oleifera seed technology, applied in the direction of oil/fat production, fat production, food science, etc., can solve the problems of flammability and explosion, residual oil in cakes, deep color, etc., and achieve the function of retaining nutrition and activity , No organic solvent residue, simple process equipment

Inactive Publication Date: 2014-05-28
NANJING FEIMA FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The pressing method mainly uses mechanical external force to squeeze the oil out of the oil. The product does not contain solvent, but the cake has a lot of residual oil, the oil output efficiency is low, and the energy consumption is large; the solvent method has a high oil output rate and is easy to realize large-scale production. However, most of the solvents used are flammable and explosive, and the production safety is poor, and the refined oil contains solvent residues, and the leached crude oil contains more non-oil substances, darker in color, and poor in quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Take 1 kg of dehulled camellia oleifera kernels and carry out dry crushing with a FW177 portable traditional Chinese medicine grinder (manufactured by Tianjin Test Instrument Co., Ltd.), mix the crushed camellia oleifera seeds with water at a ratio of 1:5, and use a JMS-50 Colloid mill (manufactured by Langfang Guantong Machinery Co., Ltd.) to grind to a particle size of 2-50 μm, add compound enzymes (4g pectinase NCB-PE40, 6g cellulase AE80), at 50°C and pH4.5, Enzymolysis was carried out for 3 hours, and then centrifuged at 40°C and 3000 rpm for 10 minutes. Take the emulsified oil, dilute it with distilled water at a ratio of 1:1, adjust the pH to 9, raise the temperature to 60°C, keep it for 40 minutes, add 2.5ml of alkaline protease Alcalase2.4L, keep it at 60°C, at about pH 9, enzymatically hydrolyze it for 60 minutes, at 50°C And under the condition of 3500rpm, centrifuge 10min, obtain camellia oleifera seed clear oil, merge with the clear oil obtained when breaki...

Embodiment 2

[0016] Take 1 kg of shelled camellia oleifera kernels and carry out dry crushing with a FW177 portable traditional Chinese medicine grinder (manufactured by Tianjin Test Instrument Co., Ltd.), mix the crushed camellia oleifera seeds with water in a ratio of 1:6, and use a JMS-50 Colloid mill (manufactured by Langfang Guantong Machinery Co., Ltd.) to grind to a particle size of 2-50 μm, add compound enzymes (5g pectinase NCB-PE40, 15g cellulase AE80), and enzymatically hydrolyze at 55°C and pH5 4h, and then centrifuged at 40°C and 3500rpm for 15min. Take the emulsified oil, dilute it with distilled water at a ratio of 1:2, adjust the pH to 11, raise the temperature to 65°C, keep it for 60 minutes, add 5ml of alkaline protease Alcalase 2.4L, keep it at 65°C, at a pH of about 11, enzymatically hydrolyze it for 100 minutes, and heat it at 60°C and Under the condition of 3500rpm, centrifuge for 15min to obtain clear oil of Camellia oleifera seed, which is combined with the clear oi...

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Abstract

The invention relates to a method for preparing highly unsaturated camellia seed clean oil by using a biological enzyme method, belonging to the field of food biotechnology. The method comprises the steps of hulling camellia seeds, drying, crushing, adding water, mixing, milling with a colloid mill till the granularity of slurry is 2-50mu m, performing enzymolysis wall-breaking through pectase NCB-PE40 and cellulase AE80 together, and centrifuging to separate clean oil and emulsified oil; demulsifying the emulsified oil by using alkaline protease Alcalase2.4L, and centrifuging to obtain clean oil; combining the clean oil obtained by demulsifying and the clean oil obtained by wall-breaking to finally obtain the highly unsaturated camellia seed clean oil. The method disclosed by the invention has the characteristics that the clean oil is extracted through wall-breaking and demulsifying by adopting the biological enzyme method, so that the process is simple, and the conditions are mild; the product has high unsaturated fatty acid content, does not have organic solvent residue and is environment-friendly.

Description

1. Technical field [0001] The invention provides a production method for preparing highly unsaturated camellia camellia seed clear oil by a biological enzymatic method, which belongs to the field of food biotechnology. 2. Background technology [0002] Camellia oleifera is an important woody oil tree species in southern my country, and is known as the world's four largest woody oil tree species together with olive, oil palm, and coconut. China is rich in Camellia oleifera resources, with a planting area of ​​4 million hectares and an annual output of 150,000 tons of Camellia oleifera seed oil, ranking first in the world. The unsaturated fatty acid content of Camellia oleifera seed oil is as high as 85%, rich in essential amino acids and trace elements such as zinc and selenium. It is a high-quality edible oil, known as "the treasure of oil", and has important development and utilization value. Commonly used methods of oil production are mainly pressing method and solvent me...

Claims

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Application Information

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IPC IPC(8): C11B1/00C11B1/04A23D9/02
Inventor 李春阳李世科杨明王艳秋
Owner NANJING FEIMA FOOD
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