Method for efficiently producing hydroxysteroid dehydrogenase with testosterone comamonas

Abstract

The invention relates to a method for efficiently producing hydroxysteroid dehydrogenase with testosterone comamonas. The method comprises steps of bacteria culture, seeding tank culture, fermentation tank culture and the like, and can produce hydroxysteroid dehydrogenase efficiently. The hydroxysteroid dehydrogenase has remarkable effects on crops in preventing disease, promoting growth and increasing yield. Furthermore, if being used as a novel fodder enzyme preparation in cultivation in stock farming, the hydroxysteroid dehydrogenase can effectively promote pigs and broiler chickens to grow, improves the laying rate of laying hens, reduces disease of beasts and birds, and has obvious effect in promoting growth. According to the method, viable bacteria in per milliliter of fermentation liquor is improved to be 30 billion from 0.1 billion, the enzyme activity in per gram of dry powder is improved to be 300 thousand from 1 thousand, so that the product cost is greatly lowered, and the method can be used for industrial production.

Description

technical field [0001] The invention relates to a method for efficiently producing hydroxysteroid dehydrogenase by Comamonas testosteroni. Background technique [0002] Any plant individual is a combination of the plant's own tissue cells, its internal and external enzymes, and microorganisms. That is to say, the enzymes and microorganisms themselves are part of the plant, and enzymes play a very important role in life activities. Enzymes can Catalyze various biochemical reactions under normal temperature and pressure, produce a variety of antibiotics and growth-promoting hormones, activate the soil to release a large amount of trace elements, increase the number of main roots and capillary roots of plants, broaden the pipeline of nutrient delivery, and allow crops to absorb in a reasonable balance Nutrients, promote crop growth, early maturity, increase income, enhance stress resistance, disease resistance, drought and flood resistance, etc. Without these enzymes, crops c...

AI Technical Summary

Problems solved by technology

[0005] At present, the phenomenon of soil self-poisoning is relatively serious. The phenomenon of self-poisoning means that some toxins, such as aldehydes, alcohols, hydrocarbons, and accumulated drug residues and steroid hormones, will be produced during the decay and decomposition process of the residues of previous crops. etc., have a significant inhibitory effect on the next stubble crops, affecting the growth of the crops themselves, coupled with the massive reproduction of soil pathogenic microorganisms, the number is increasing year by year, due to the weak resistance of the seedlings, they are prone to disease, the deficiency or imbalance of nutrient elements, soil nutrition Physiological imbalance of elements will directly cause crop nutrition imbalance, crop root rot and underdeveloped root system, which will affect the normal physiological metabolism of crops, resulting in large-scale production reduction or even failure of crops