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Method for producing organic selenium-enriched lactic acid bacteria

A technology of lactic acid bacteria and organic selenium, applied in the direction of microorganism-based methods, biochemical equipment and methods, medical preparations containing active ingredients, etc., can solve the problems of unsatisfactory transformation and utilization of inorganic selenium, lack of biological functions of selenium, etc. Achieve the effects of intervening in the lipid peroxidation reaction of liver fibrosis tissue cells, improving nutrition and medical care functions, and increasing added value

Inactive Publication Date: 2014-05-21
QINGDAO ANBEIKANG BIOLOGICAL MEDICINE TECH CO LTD
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  • Application Information

AI Technical Summary

Problems solved by technology

However, the selenium content of selenium-enriched yeast is usually 0.3 mg / g, up to 1 mg / g (Acta Microbiology, 1990, 30 (1): 36-40; Shanxi Food Industry, 1999, (2): 28-29). The conversion and utilization of inorganic selenium is still not ideal
Lactic acid bacteria are well-known strains for food processing and preservation. They can secrete various enzymes, provide rich amino acids and vitamins, and have biological functions such as antibacterial, lowering cholesterol, maintaining micro-ecological environment, anti-tumor, and enhancing immunity. Does not have the biological function of selenium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment l

[0015] Example 1: Preparation of lactic acid bacteria MRS liquid medium: add peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 20.0g, sodium acetate 5.0g, citrate triamine 2.6g, Tween 801.0 into 1000ml of distilled water g, K 2 HPo 4 2.0g, MgSo 4 ·7H 2 o0.58g, MnSo 4 ·H200.25g; mix and dissolve, adjust the pH to 6.0-6.4, and sterilize at 121°C for 15 minutes.

[0016] Take the activated Lactobacillus bulgaricus colony in MRS liquid medium, culture and expand at 42℃, add 5% sodium selenite solution to make the selenium content of the medium 64mg / L, continue to cultivate for 24h, collect the bacterial liquid , Centrifugation (2000r / min, 15min), repeated washing with sterile physiological saline for 5 times, and then centrifugation, the precipitate is the Lactobacillus bulgaricus preparation rich in organic selenium.

[0017] Determine the selenium content according to the following method:

[0018] (1) Preparation of standard selenium working solution

[0019] Precisely we...

Embodiment 2

[0024] Example 2: Basically the same as Example 1, except that the colonies of activated Streptococcus thermophilus were cultured and amplified at 42°C for 12 hours in the lVIRS liquid medium, and 8% sodium selenite solution was added to make The selenium content of the culture medium is 30me / L. Continue to culture for 12h, collect the bacterial liquid, centrifuge (2000r / min, 15min), and wash 3 times with distilled water. After centrifugation, the sediment is the organic selenium-rich Streptococcus thermophilus Preparation, organic selenium content can reach 6-12me / g.

Embodiment 3

[0025] Example 3: Basically the same as Example 1, except that the Lactobacillus bulgaricus was replaced by Lactobacillus acidophilus. After 24 hours of culture and expansion, 9% sodium selenite solution was added to make the selenium content of the medium 120me / r, continue culturing for 36h, collect the bacterial liquid, centrifuge (2000r / min, 15min), and wash 3 times with physiological saline. After centrifugation, the supernatant is removed to obtain a Lactobacillus acidophilus preparation rich in organic selenium. The organic selenium content can reach 8-15mg / g.

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Abstract

The invention relates to the field of biology and in particular discloses a method for preparing organic selenium-enriched lactic acid bacteria. The method is characterized by comprising the following steps: a, inoculating lactic acid bacteria to a culture medium in a conventional mode, and performing culture amplification for 24-36 hours; b, adding a sodium selenite solution with the mass concentration of 5-8 percent into fermentation liquor in the step a, so that the selenium content of the fermentation liquor is 30-90mg / L, and continuously culturing at a constant temperature for 24-36 hours; and c, centrifuging the bacteria liquid obtained in the step b, washing for 5 times by using stroke-physiological saline solution, centrifuging, removing the supernatant, and obtaining the organic selenium-enriched lactic acid bacteria preparation. According to the production method, the oxidation resistance and the immunologic function of the normal organism can be improved, the lipid peroxidation reaction of hepatic fibrosis cells is intervened, and particularly, the advantages of an antioxidant enzyme activity system are obviously improved.

Description

Technical field [0001] The invention relates to the field of biological products, in particular to a method for producing selenium-enriched lactic acid bacteria preparations. Background technique [0002] Selenium is an essential trace mineral nutrient for the human body. The total selenium content of the human body is 6-20mg. Selenium can be found in various tissues, organs and body fluids, with the highest concentration in the kidney, which is very important for improving immunity and preventing cancer. Studies have shown that selenium is an essential component of glutathione peroxidase (gSH-Px), which the body relies on to remove lipid peroxides and free radicals. One of the important mechanisms for selenium is through GSH. -Px antioxidant activity is achieved. Selenium can prevent liver disease by improving the GSH-Px activity of liver cells and Ito cells, preventing lipid peroxidation damage. Liver fibrosis is a chronic liver injury response of the liver to various stimul...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/74A61P1/16A23L1/29C12R1/225C12R1/46C12R1/23C12R1/01A23L33/00A61K35/744
Inventor 不公告发明人
Owner QINGDAO ANBEIKANG BIOLOGICAL MEDICINE TECH CO LTD
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