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Aqueous extraction method of hydroxytyrosol from inca inchi leaves

A technology of hydroxytyrosol and sacha inchi, which is applied in chemical instruments and methods, preparation of organic compounds, organic chemistry and other directions, can solve problems such as no literature reports on extracting hydroxytyrosol, and achieves low production cost, reasonable extraction process, Simple to use effects

Active Publication Date: 2015-07-15
上海韵尔化妆品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] According to the novelty search by Hainan Provincial Institute of Medical Information, there is no literature report on the extraction of hydroxytyrosol from Sacha Inchi leaves

Method used

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  • Aqueous extraction method of hydroxytyrosol from inca inchi leaves
  • Aqueous extraction method of hydroxytyrosol from inca inchi leaves
  • Aqueous extraction method of hydroxytyrosol from inca inchi leaves

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experiment example 1

[0024] Investigation on Extraction Process and Purification Method

[0025] Based on the consideration of the better water solubility of hydroxytyrosol, the water-soluble method was directly used for extraction, and factors such as soaking time, extraction temperature, amount of water added, extraction time, extraction times, and purification methods were comprehensively investigated. Each factor was investigated. levels. conclusion as below:

[0026] (1) Soaking time factor

[0027] Take 100 g of dried Sacha Inchi leaves (the content of hydroxytyrosol is 0.8%) and add 2000 mL of water, respectively soak for 0.5, 1, 2, 3, 4 h, extract 2 h in a cycle, and the extract is centrifuged (≥2500 rpm , 10 min), the precipitate was discarded, and the obtained supernatant was concentrated to dryness under reduced pressure at 60°C, and the product was collected, weighed, and determined. The results are shown in Table 1.

[0028] When soaking (h) 0 0.5 1 2 3 4 Yiel...

experiment example 2

[0083] Determination of hydroxytyrosol content (HPLC method)

[0084] (1) Chromatographic conditions

[0085] LC-2010AHT High Performance Liquid Chromatograph

[0086] Chromatographic column: Diamonsil C18 (250 mm×4.6 mm, 5 μm)

[0087] Mobile phase: methanol-0.4% acetic acid aqueous solution (10:90)

[0088] Flow rate: 1.0 mL / min,

[0089] Detection wavelength: 280 nm,

[0090] Column temperature: 30°C

[0091] Determination results of hydroxytyrosol content in raw material (inchi leaf)

[0092] The content of hydroxytyrosol in the raw materials dried in the shade is 0.7%; the content of hydroxytyrosol in the raw materials dried in the oven at low temperature (below 50°C) is 0.9%.

[0093] The results showed that the content of hydroxytyrosol in the natural shade-dried raw materials was slightly lower than that in the dried raw materials. Therefore, it is better to dry the raw materials.

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Abstract

The invention relates to the plant extraction field and relates to an aqueous extraction method of hydroxytyrosol from inca inchi leaves. The method comprises the following steps: (1) adopting inca inchi leaves as raw materials; (2) performing aqueous extraction twice, combining extracted solutions, and centrifuging to remove impurities, adsorbing clear solution through the D-101 resin, mixing eluent, performing reduced-pressure distillation at 60 DEG C, recycling ethyl alcohol, and then freezing and drying the ethyl alcohol after the ethyl alcohol is continuously evaporated and concentrated to be 1.05g / mL. Water is used as the extracting solution, the production cost is greatly reduced, high safety is achieved, the extraction process is reasonable, the operation is simple, and the impurities in the extracts are less. The yield of extract is 2.5%-3.1%, the content of the hydroxytyrosol is 25%-30%, the extraction rate is high, and the extraction ratio of the effective constituent is 82%-85%.

Description

[0001] technical field [0002] The invention relates to the field of plant extraction, namely a method for water extraction of hydroxytyrosol in sacha inchi leaves. Background technique [0003] In the prior art, hydroxytyrosol (English: hydroxytyrosol), chemical name: 3,4-dihydroxyphenethyl alcohol, molecular formula: C 8 h 10 o 3 , Solubility: soluble in alcohol, water, and lipid solvents. [0004] The efficacy of hydroxytyrosol: (1) anti-tumor. (2) Antithrombotic, regulating blood fat and anti-arteriosclerosis. (3) Anti-pathogenic microorganisms. (4) Prevention and treatment of macular degeneration. (5) Inhibit DNA oxidative damage and protect skin from photodamage. (6) Anti-inflammatory and analgesic. (7) Cartilage protection and anti-osteoporosis effects. (8) Others: It can effectively reduce and inhibit the harm of smoking to the human body; quickly restore physical fitness, enhance resistance and exercise ability; lose weight, etc. [0005] The scope of appl...

Claims

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Application Information

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IPC IPC(8): C07C39/11C07C37/70C07C37/82
CPCC07C37/004C07C39/11
Inventor 胡思宁王勇肖茜莹肖培富孙炎
Owner 上海韵尔化妆品有限公司
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