Method for acquiring novel species of brassicaceous vegetables and application method of novel specie of brassicaceous vegetables

Abstract

The invention discloses a method for acquiring novel species of brassicaceous vegetables and an application method of the novel species of the brassicaceous vegetables. According to the method, free microspore culture is carried out between hybrid seeds of the brassicaceous vegetables of 'celery cabbage species*small cabbage species' or 'small cabbage species*variants' to obtain regenerated plants so as to create the novel species. The method is applied to breeding and comprises the main steps of carrying out vegetable hybridization on 'celery cabbage species*'small cabbage species' and 'small cabbage species*variants', inducing embryoid by virtue of the free microspore culture, acquiring the regenerated plants through the germination of embryoid, carrying out ploidy determination on the regenerated plants acquired through the culturing of the microspore culture, reserving seeds of double haploid and high haploid, carrying out morphological identification and nutritive index identification on descendants of the planted regenerated plants, and preparing cross composition. The method has the advantages of short period and high efficiency, provides materials for the efficient breeding of Chinese cabbage vegetables and has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of biotechnology and haploid breeding, and in particular relates to a method for quickly obtaining new germplasm of cruciferous vegetables through a free microspore culture method and applying it to breeding. Background technique [0002] Cruciferous (2n=2x=20) vegetables are divided into pakchoi subspecies and Chinese cabbage subspecies. Yongcai, etc., this subspecies is characterized by open leaves, short plant type, and obvious petioles. Most of the leaves are smooth and hairless, without obvious leaf wings, and do not form obvious leaf bulbs. The subspecies of Chinese cabbage can be divided into loose-leaf varieties, semi-headed varieties, flower-centered varieties, and head-shaped varieties. They are characterized by larger plant types, obvious petioles and leaf wings, and many rough leaf surfaces. Form leaf balls. There are great differences in vegetable traits between subspecies and varieties within...

AI Technical Summary

Problems solved by technology

The advantages of different subspecies and varieties of vegetables can be combined through sexual hybridization. The hybrid offspring are highly heterogeneous in genotype, and the highly pure parents required in dominant hybrid breeding can be self-crossed and segregated by conventional methods. Continue self-breeding for several generations, labor-intensive and time-consuming

With the improvement of people's living standards, the traditional method of hybridization within varieties and between varieties is difficult to meet the requirements of high-yield, high-quality, and novel-shaped vegetables required by the market, and the hybrids between subspecies and within subspecies are free Microspore culture can quickly obtain double haploid pure lines, and the differences between the pure lines are very large, which provides a larger selection range, solves this problem, and meets the needs of breeding work

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