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A strain of Cordyceps sinensis with high sporulation

A technology of Cordyceps sinensis fungi and strains, applied in the directions of fungi, methods of using spores, microorganisms, etc.

Inactive Publication Date: 2015-10-28
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The strain has been preserved in the General Microorganism Center of China Committee for the Collection of Microbial Cultures (CGMCC for short) on September 4, 2012.

Method used

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  • A strain of Cordyceps sinensis with high sporulation
  • A strain of Cordyceps sinensis with high sporulation
  • A strain of Cordyceps sinensis with high sporulation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1. Isolation and biological characteristics of Cordyceps 1621

[0033] 1. Isolation of Cordyceps 1621

[0034] The inventor of the present application screened strains from five provinces and regions where Cordyceps sinensis is produced in the country from the Cordyceps species library that has been identified in the laboratory, and obtained the dominant strain 1621. After liquid culture, the dry weight of mycelium and the culture medium The test performance of the two indicators of utilization (residual sugar) is good; after solid culture, the test of 1621 bacteria producing conidia performed well. For the test method, see [Yao Yijian, Ren Shuyu. A method for preparing cordyceps fungus conidia inserts and dynamically counting sporulation. Patent application number: 201110055807.1; Yao Yijian, Ren Shuyu, Wang Wenjing. A method for preparing a cordyceps fungus conidia cover sheet and dynamically counting the sporulation. Patent application number: 201110054454.3]. ...

Embodiment 2

[0043] Example 2. Spore production of Cordyceps 1621

[0044] Strains in this embodiment: Cordyceps sinensis 1621 and Cordyceps 762

[0045] 1. Preparation of bacterial seed liquid

[0046] The cordyceps fungus was inoculated into the above solid medium and cultured at 15°C for 90 days to obtain the cordyceps fungus strain. Wash the Cordyceps strain plate with sterile water, and inoculate the washed bacterial suspension into the above liquid medium at an inoculum volume of 20% by volume. The volume of the bottle is 1 / 5 of the volume of the Erlenmeyer flask, then at 18°C, Rotate speed 100rpm, 24 hours of dark conditions, shake culture for 14 days to obtain the first strain of Cordyceps sinensis; inoculate the first strain of Cordyceps sinensis in a new liquid medium at a volume ratio of 10%, and other cultures Under the same conditions, the second strain of Cordyceps sinensis was obtained.

[0047] 2. Detection of sporulation time

[0048] Inoculate the obtained 0.5 mL of the second b...

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Abstract

The invention discloses an Ophiocordyceps sinensis strain with high spore yield. The Ophiocordyceps sinensis strain with high spore yield provided by the invention is Ophiocordyceps sinensis 1621, the registered number of which in the China General Microbiological Culture Collection Center is CGMCC No.6445. The Ophiocordyceps sinensis 1621 has a fast mycelium growth speed, short conidiospore generation time and high spore yield.

Description

Technical field [0001] The invention relates to a strain of Cordyceps sinensis with high spore production. Background technique [0002] Cordyceps sinensis [Ophiocordyceps sinensis (Berk.) G. H.Sung, J.M.Sung, Hywel-Jones & Spatafora (≡Cordyceps sinensis (Berk.)Sacc.)] is a rare and precious medicinal fungus unique to the Qinghai-Tibet Plateau. Modern research shows that Cordyceps has a variety of pharmacological effects and has a good application prospect. Although a lot of research work has been done on Cordyceps sinensis, there are still obvious technical problems in artificial cultivation. Solving these problems requires a more comprehensive understanding of the biological characteristics of Cordyceps. Therefore, the innovation of biological research technology of Cordyceps sinensis has important theoretical and practical significance. [0003] In the study of Cordyceps fungus culture, the study of conidia is closely related to the preparation of fungus. At present, the mai...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N3/00C12R1/645
Inventor 姚一建任蜀豫王晓亮
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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