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Vaccinium bracteatum leaf pigment extraction process

A technology of the leaves and extraction process of black rice, applied in the field of high-efficiency extraction and preparation of leaf pigments of black rice, can solve the problems of reduced adsorption capacity, pollution, particle breakage and the like

Inactive Publication Date: 2014-04-30
QINGDAO ZHONGREN ZHIYE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] From the point of view of the purification process after pigment extraction, most of the pigments are purified by macroporous resins at present. This method has the following problems: (1) The safety of the resin mainly refers to the safety brought by porogens and degradation products Problems; (2) The adsorption capacity is reduced when used repeatedly; (3) The particles may be broken during the use of the resin; (4) The separation capacity is reduced by contamination by other substances

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A process for extracting black rice leaf pigment, it comprises the following steps:

[0029] (A) Pulverization: take Yixing fresh Wufan leaves as raw materials, wash them with water, and then mechanically pulverize them for later use;

[0030] (B) Extraction: add water to the pulverized black rice leaf residue according to the ratio of solid to liquid 1:10 (g / mL), and set the ultrasonic-microwave-assisted extraction parameters: ultrasonic frequency is 40KHz, extraction temperature is 40°C, microwave power 95W, extraction time 20min, ultrasonic-microwave assisted extraction is performed under this parameter;

[0031] (C) Centrifugation: Refrigerate and centrifuge at a centrifugation speed of 10,000 rpm and a centrifugation time of 20 minutes to remove the precipitate and take the supernatant for use;

[0032] (D) Purification and concentration: Purify and concentrate the supernatant under the conditions of a ceramic membrane with a pore size of 150nm and an operating pr...

Embodiment 2

[0035] A process for extracting black rice leaf pigment, it comprises the following steps:

[0036] (A) Pulverization: take Yixing fresh Wufan leaves as raw materials, wash them with water, and then mechanically pulverize them for later use;

[0037] (B) Extraction: add water to the crushed black rice leaf residue according to the ratio of solid to liquid 1:15 (g / mL), and set the ultrasonic-microwave-assisted extraction parameters: ultrasonic frequency is 40KHz, extraction temperature is 50°C, microwave power 90W, extraction time 25min, ultrasonic-microwave assisted extraction is performed under this parameter;

[0038] (C) Centrifugation: Refrigerate and centrifuge at a centrifugation speed of 9000rpm and a centrifugation time of 25min to remove the precipitate and take the supernatant for use;

[0039] (D) Purification and concentration: Purify and concentrate the supernatant under the conditions of a ceramic membrane with a pore size of 160nm and an operating pressure of 0...

Embodiment 3

[0042] A process for extracting black rice leaf pigment, it comprises the following steps:

[0043] (A) Pulverization: take Yixing fresh Wufan leaves as raw materials, wash them with water, and then mechanically pulverize them for later use;

[0044] (B) Extraction: add water to the pulverized black rice leaf residue according to the ratio of solid to liquid 1:20 (g / mL), and set the ultrasonic-microwave-assisted extraction parameters: ultrasonic frequency is 40KHz, extraction temperature is 55°C, microwave power 85W, extraction time 30min, ultrasonic-microwave assisted extraction is performed under this parameter;

[0045] (C) Centrifugation: Refrigerate and centrifuge at a centrifugation speed of 8000 rpm and a centrifugation time of 30 minutes to remove the precipitate and take the supernatant for use;

[0046] (D) Purification and concentration: Purify and concentrate the supernatant under the conditions of a ceramic membrane with a pore size of 180nm and an operating pres...

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Abstract

The invention relates to a vaccinium bracteatum leaf pigment extraction process, which comprises: cleaning vaccinium bracteatum leaves with water, carrying out mechanical crushing, adopting water as an extraction solvent, adopting an ultrasound-microwave assisted extraction technology to extract, carrying out freezing centrifugation on the extraction solution, removing the precipitate, taking the supernatant, carrying out micro-filtration purification with a ceramic membrane, concentrating the supernatant, and carrying out freeze-drying on the concentrated solution to prepare the vaccinium bracteatum leaf pigment. According to the present invention, with the method, the yield and the purity of the vaccinium bracteatum leaf pigment can be substantially increased; the extracted vaccinium bracteatum leaf pigment has characteristics of good safety and strong biological activity; and the extraction process has characteristics of simpleness, short time, low energy consumption, low cost, high efficiency and green production, and is suitable for industrial production.

Description

technical field [0001] The invention relates to the field of extraction of natural pigments, in particular to a method for efficiently extracting and preparing the pigments from leaves of black rice leaves. Background technique [0002] The black rice tree plant belongs to Rhododendronaceae, and has a long history of application in our country, whether it is used as food or medicine, it is very distinctive. There is almost no research on the leaves of the black rice tree abroad, and there have been research reports on the extraction of pigments from the leaves of the black rice tree. Wang Li et al. used pectinase and cellulase to study the extraction of melanin from the leaves of the black rice tree under buffer conditions, and found that the combination of pectinase and cellulase is beneficial to improve the yield of melanin. Xu Lei from Henan University of Technology, Wang Li from Jiangnan University, Hu Zhijie from Nanjing Forestry University, Jiang Ping and others used ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00
Inventor 谭斌峰
Owner QINGDAO ZHONGREN ZHIYE BIOTECH
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